The Article gives information on the meeting of CIS Intergovernmental Council for Collaboration in the Field of Veterinary Medicine which was held in Sochi, February 19-20, 2015. Different issues related to joint actions of the CIS Veterinary Services were discussed at the meeting.

The information on the establishment of the FGBI «ARRIAH» Branch in the Republic of Crimea and stages of its development is given in the paper. Some aspects of its activities are under review and obtained results are analyzed.

The article presents information about the personnel of the FMD Research Institute and FGBI «ARRIAH» - veterans of the Great Patriotic War, home-front workers and children born during the war.

A capability to adapt Schmallenberg virus to continuous cell cultures of different origin was investigated. The evidence of virus adaptation was established in continuous cell cultures BHK-21, porcine kidney, Vero, ovine kidney and Mark-145. The more efficient cell cultures were ВНК-21, PK and Vero. The aforementioned cell cultures could be of interest when choosing sensitive systems for cultivation of the agent for the purpose of receiving active virus-containing material.

In recent decades the contraction of necrobacillosis by cattle is a serious inhibitor on the way of successful development of dairy and beef raising. All hopes of the disease prevention, as the disease is infectious one, are focused on veterinary science and activities of veterinarians. Guided by the recognized insight into infectious pathology they make many efforts to develop different medicinal drugs for its treatment and specific prevention. However, the level of necrobacillosis incidence among production animals does not decrease but even more and more increases from year to year.

A molecular cloning of African swine fever virus k205r and b602l genes in £ coli was carried out. The expression and purification conditions ensuring high yield of recombinant proteins were optimized. Dissolved recombinant proteins were purified by metal-chelate affinity chromatography using Ni-NTA-agarose («Qiagen»). Recombinant antigens are biologically safe, easier to prepare and ensure higher ELISA specificity. The purified protein yield from 100 ml off. coli culture was 1,5 mg for pK205R and 2 mg for pB602L.

The data on studying immunogenic activity of the inactivated vaccine produced on the basis of avian adenovirus isolate FAdV-2 are demonstrated in the paper. The dynamics of antibody accumulation in chicken blood is shown. The high resistance of immunized chicks to homologous virus (FAdV-2) infection and lack of protection against heterologous virus (FAdV-4) infection are also demonstrated.

Method of identification ofT- and B-lymphocytes in chickens and their subpopulations by flow cytofluorometric analysis using BD FACSVerseTM was developed. Method of lymphocytic gate extraction with control of leukocyte common antigen CD45 and monocytic antigen expression was determined. Antibody panels for immunophenotyping lymphocytes when testing chicken cell mediated immune response were selected and tested. They enable to determine a relative number of T- and B-lymphocytes, T-helpers and T-cytotoxic cell populations in blood and chicken lymphoid organs. The paper demonstrates specificity and reproducibility of the developed method of identification of chicken lymphocyte subpopulation.

Latex-agglutination preparations were produced for detection of antibodies to infectious hematopoietic necrosis virus of salmon with analytical sensitivity of 0,9 цд/ті. While testing 40 blood samples using latex-agglutination test the diagnostic sensitivity and specificity were 92 and 100%, respectively. The latex-agglutination is a simple and rapid test; it can be used for retrospective diagnostics of the disease and determination of the titer of antibodies to the virus under conditions of a veterinary laboratory and even a fish farm.

The epidemic situation on aquaculture diseases worldwide is of relevance for our country as this information makes it possible to imagine a risk of infection spread among countries and to determine effectiveness of necessary measures aimed at prevention of these diseases spread in territories of neighboring and other countries. The epidemiological monitoring is the basis for rational planning and effective arrangement of measures for controlling fish infectious diseases.