INDIRECT ELISA FOR DETECTION OF ANTIBODIES TO FMDV NON-STRUCTURAL PROTEINS IN PORCINE SERA
https://doi.org/10.29326/2304-196X-2018-3-26-13-16
Abstract
An indirect variant of ELISA used for detection of antibodies to nonstructural proteins of the FMD virus in porcine blood sera was developed. The results of the validation showed that the developed method is characterized by high sensitivity, specificity and reproducibility. When testing the blood serum panel obtained from experimentally infected animals, the method allowed to detect antibodies to FMD virus in 7 of 18 sera collected on day 6 post inoculation, in 13 of 19 sera – on day 7 post inoculation, in 16 of 19 sera – on day 8 post inoculation and in all 76 sera obtained on days 9–12 post inoculation. The diagnostic specificity of 3AB-ELISA was 100% when testing 100 knowingly negative blood sera from pigs imported to Russia from Norway. High specificity and sensitivity of the method, established during the development of the method, are confirmed in the course of routine diagnostic tests.
About the Authors
A. S. Yakovleva
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
Senior Researcher, Candidate of Science (Biology),
Vladimir
A. V. Kanshina
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
Senior Researcher, Candidate of Science (Veterinary Medicine),
Vladimir
A. V. Scherbakov
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
Head of Laboratory, Candidate of Science (Biology),
Vladimir
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Review
For citations:
Yakovleva A.S., Kanshina A.V., Scherbakov A.V. INDIRECT ELISA FOR DETECTION OF ANTIBODIES TO FMDV NON-STRUCTURAL PROTEINS IN PORCINE SERA. Veterinary Science Today. 2018;(3):13-20. https://doi.org/10.29326/2304-196X-2018-3-26-13-16
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