The paper demonstrates results of long-term monitoring research performed by the FGBI "ARRIAH" in the Russian part of Uvs-Nuur Lake water area in the Republic of Tyva. The monitoring was aimed at the detection of influenza A virus circulation in the population of wild migratory birds. Ecological and biological significance of the lake coastal area in the arrangement of epidemiological investigations was determined. Highly pathogenic avian influenza virus circulation was identified in populations of wild avifauna in Uvs-Nuur migration area.

Test system based on the immunoenzyme method was developed for detection of antibodies to the avian leukosis virus (ALV) when testing sera in a single dilution. The antibody titer was determined basing on S/P ratio calculated for one working serum dilution (1:400). The developed test system demonstrated high specificity and sensitivity in comparison with the foreign commercial kit.

Experimental infection of 7-day-old chicks with the isolate of avian metapneumovirus Subtype В induced mild signs of respiratory infection and swelled infraorbital sinuses in 5-15 days post infection. Lesions in tissues of trachea, nasal passages and primary lymphoid organs were reported. Using real-time RT-PCR avian metapneumovirus genome was detected in the upper part of the respiratory tract, infraorbital sinuses and Harderian gland within 2-12 days post infection. The immune response was characterized by formation of humoral and local antibodies in 7-21 days post infection. In 3 days post infection increased percentage of CD8a+ T-cell subpopulation was reported in blood and spleen of the infected chicks.

The paper demonstrates results of the research aimed at optimization of amino acid composition of the nutrient media used for BHK-21-2/17 suspension cultivation by change of amino acid amount in such media. Growth level in the control and tested media amounted to 5.6; cell growth dynamics was also similar. In-media utilization of different amino acids was different. The cells mostly consumed glutamin, serine, tyrosine, methionin (up to 90%, 55%, 40% and 60%, respectively). Growth of alanine level was reported (from 10% in the control sample and up to 60% in the test sample). Amount of tryptophan and glutamic acid remained stable. Glycine, threonine, isoleucine were actively utilized within the first 24 hours (7-13%, 10-18%, 34-41%, respectively in the control experiment). Hereafter, the concentration slightly increased (by 7-45%, by 16-21%, by 20-50%, respectively in the control experiment). During the FMDV reproduction in the cells grown in the test and control media no significant differences in the yield of immunogenic virus components were reported.

The paper presents analytical review of data concerning the reasons for mass deaths of saiga antelopes in Kazakhstan. It was demonstrated that the data on saiga antelope disease etiology are contradictory: pasteurellosis (bovine haemorrhagic septicemia), anaerobic enterotoxemia, ruminai tympany, tryptophan-induced bovine atypical interstitial pneumonia and unknown virus. It means that when investigating the reasons of mass morbidity and mortality in wild ruminants the data of epidemic survey, remote clinical examination, post mortem lesions and results of laboratory tests of pathological samples from dead and killed animals were taken into account. To study the reasons of mass deaths in wild ruminants it is necessary to involve highly qualified specialists experienced in this pathology research. It was shown that clinical and pathological samples collected from dead and killed wild animals shall be tested according to the procedure of precise testing centres also for the purposes of excluding infections pathology and intoxications of different nature.

The paper presents the study results of sensitivity to lumpy skin disease virus of cattle and sheep experimentally infected with "E-95" virulent strain. It was established that cattle is more sensitive to LSDV in comparison to sheep: the disease in cattle was severe with fever, anorexia, cachexia and nodule development. Sheep reacted to virus inoculation with the development of nodules and swelling in the site of virus suspension injection, conjunctivitis, short-term anorexia and fever during 3-5 days post infection. The disease specificity was confirmed by PCR and prolonged complement fixation test.

The results of comparative testing of the following two vaccines produced by the FGBI "ARRIAH": inactivated emulsion marked-strain based vaccine against Aujeszky's disease in pigs and dry cultural marked-BK strain virus vaccine against Aujeszky's disease in pigs and sheep are presented.The results of immunogenic property study after challenge are given.

The paper deals with the issue of control strategies against porcine reproductory and respiratory syndrome in the Russian Federation. Biological and legal factors able to influence the quality of strategies applied in the RF are considered. It is recommended to develop a comprehensive approach to PRRS diagnostics, control and prevention for pig industry and update national PRRS legal and regulatory framework in the RF.

Aquaculture is a fast growing industry, which produces today more than 30 species of fish. The growth of aquaculture in the last decades has been supported by the development of oil adjuvanted injectable vaccines that allowed a long term protection offish and a strong reduction of the use of antibiotics. Today, injectable vaccines for fish are administered through intraperitoneal injection and are usually adjuvanted with water in oil emulsion adjuvants. Montanide“ ISA 763 AVG is a non mineral oil based adjuvant which has been extensively used for vaccination of diverse fish species (salmon, trout, seabass, tilapia, ect.). Other routes of administration such as immersion and oral administration are also considered and new adjuvants and formulations are being developed for these applications.

The paper presents data on microflora species and population analysis for feline sepsis treatment efficiency control using different schemes. Sepsis diagnostics demonstrated a significant (by 37.7%) prevalence of gram-negative microorganisms compared to gram-positive ones as well as the presence of Candida species which is indicative of severe purulent inflammation in experimental cats. The provided data also suggest that in the course of sepsis treatment intestinal microbe ecosystem stabilizes more effectively and tends towards healthy cat parameters in B₃ group animals. On the seventh day of treatment the cats from the abovementioned group showed a significant decrease in the number of Staphylococcus, Streptococcus, Escherichia, Pseudomonas, Klebsiella, Citrobacter, Proteus and Candida species in their feces samples in comparison to samples tested before treatment. This happened in parallel to a significant increase in the number of probiotic microflora species, like Lactobacillus and Bifidobacterium in comparison to initial results. It was established that sepsis treatment was most effective in Group B₃ cats where in average incarnations appeared 6.19 days earlier than in animals of B₁ Group, primary lesions healed 9.91 days earlier and the total clinical improvement occurred 5.78 days earlier.