TESTING SHEEP AND GOAT POX VIRUSES FOR THEIR REPRODUCTION IN PRIMARY AND SUBCULTURED CELLS

Results of tests of sheep and goat poxviruses for their reproduction in primary and subcultured cell cultures derived from lamb and goat kid kidneys and testicles are presented. Monolayer cultures were subcultured by 5 passages in plastic vials and infected with sheep and goat poxviruses. It was shown that production ARRIAH strain of sheep pox virus and ARRIAH 2003 strain of goat pox virus successfully propagated both in primary lamb and goat kid kidney and testicle cell cultures and lamb and goat kid kidney and testicle cell subcultures. Activity of sheep and goat poxviruses passaged 5 times was 5.5–6.0 lg TCID50/cm3. Taking into account that modern cell cultivation conditions allow primary trypsinized cell populations subcultivation up to 25–30th passage, subcultures together with continuous cell lines can be used for large-scale sheep and goat poxvirus production and research purposes.

1. Viral animal diseases [Virusnye bolezni zhivotnyh]. V. N. Syurin, А. Ya. Samuylenko, B. V. Solovyev, N. V. Fomina. М.: VNIITIBP, 1998 (in Russian).

2. Grachyov D. V. Immunobiological properties of goat poxvirus isolated in the Republic of Tajikistan [Immunobiologicheskie svojstva virusa ospy koz, vydelennogo v Respublike Tadzhikistan]: author’s abstract, Theses of Candidate of Science (Veterinary Medicines). Pokrov, 2006 (in Russian).

3. Diev V. I., Zakharov V. M., Rakhmanov A. M. Sheep and goat pox: disease spread monitoring and prevention [Ospa ovec i koz: monitoring rasprostraneniya i profilaktika]. Monitoring rasprostraneniya i predotvrashcheniya osobo opasnyh boleznej zhivotnyh: Proceedings of the 2nd International Scientific Conference. Samarkand, 2004; 63–65 (in Russian).

4. Diev V. I., Sokolov L. N., Gusev А. А. Immunobiological properties of vaccine sheep poxvirus [Immunobiologicheskie svojstva vakcinnogo shtamma virusa ospy ovec]. Problemy infekc. patologii s.-h. zhivotny: reports’ theses. Vladimir, 1997; 81 (in Russian).

5. Ivanyuschenkov V. N., Kekukh V. G., Koreba О. А. Reactogenic and immunogenic properties of anti-sheep poxvirus vaccine based on NISkHI strain [Reaktogennye i immunogennye svojstva virusvakciny protiv ospy ovec iz shtamma NISKHI]. Veterinariya. 1990; 7: 28–30 (in Russian).

6. Kukushkina M. S. Immunobiological characterization of vaccine and virulent strains of sheep and goat poxviruses [Immunobiologicheskaya harakteristika vakcinnyh i virulentnyh shtammov virusov ospy ovec i ospy koz]: author’s abstract, Theses of Candidate of Science (Biology). Vladimir, 2008 (in Russian).

7. Cultivation of VNIIZZH strain of sheep poxvirus in continuous cell lines [Kul’tivirovanie virusa ospy ovec shtamma «VNIIZZH» na perevivaemyh liniyah kletok]. Ye. V. Kurnenkova, V. I. Diev, B. L. Manin, V. P. Melnikov. Rasprostranenie i mery bor’by osobo opasnyh boleznej zhivotnyh i ptic: Proceedings of the 5th International Scientific Conference. Samarkand, 2016; 144–145 (in Russian).

8. Segeyev V. А. Propagation of animal viruses in tissue culture [Razmnozhenie virusov zhivotnyh v kul’ture tkani]. М.: Kolos, 1966 (in Russian).

9. Sheep and goat pox epidemic situation and prevention in Russia [Epizooticheskaya situaciya po ospe ovec i koz i ee profilaktika v Rossii]. V. М. Zakharov, V. I. Diev, A. M. Rakhmanov, N. А. Yaremenko. Veterinarnye i medicinskie aspekty zooantroponozov: Proceeding of International Scientific and Practical Conference devoted to the 45th anniversary of the Institute. Pok¬rov, 2003; 2: 345–354 (in Russian).

10. Animal Health Status and Disease Control Methods in Member Countries in 2000. OIE. Paris, 2001; Parts 1–3.

11. Davies F. G., Otema C. Relationship of capripox viruses found in Kenia with two Middle Eastern strains and other orthopox viruses. Res. Vet. Sci. 1981; 31 (2): 253–355; PMID: 7034101.

12. Kitching R. P., Taylor W. P. Clinical and antigenic relationship between isolates of sheep and goats pox viruses. Trop. Anim. Health. Prod. 1985; l7 (2): 64–74; DOI: 10.1007/BF02360774.

13. Sheep pox and goat pox. OIE. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees). 2018. Chap. 3.7.12; URL: http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/3.07.12_S_POX_G_POX.pdf.

14. World Animal Health in 2003. OIE. Paris, 2004; Vol. 2.