DEVELOPMENT AND VALIDATION OF 3AB-ELISATEST-SYSTEM FOR DETECTION OF ANTIBODIES TO FMD VIRUS NONSTRUCTURAL PROTEINS IN BLOOD SERA FROM CATTLE AND SMALL RUMINANTS
Abstract
The 3AB-ELISA test-system for detection of antibodies to FMD virus nonstructural proteins in blood sera from cattle and small ruminants was validated. It was discovered that the reaction repeatability and reproducibility exceeded 90%, diagnostic specificity constituted 99,8%, diagnostic sensitivity came up to 96,6%. The high specificity and sensitivity of the 3AB-ELISA test-system was confirmed by international proficiency testings as far as FMD diagnostics and by routine diagnostic and monitoring studies.
About the Authors
A. S. Yakovleva
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
A. V. Kanshina
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
A. V. Scherbakov
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
Ye. S. Orlova
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
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Review
For citations:
Yakovleva A.S., Kanshina A.V., Scherbakov A.V., Orlova Ye.S. DEVELOPMENT AND VALIDATION OF 3AB-ELISATEST-SYSTEM FOR DETECTION OF ANTIBODIES TO FMD VIRUS NONSTRUCTURAL PROTEINS IN BLOOD SERA FROM CATTLE AND SMALL RUMINANTS. Veterinary Science Today. 2015;(4):36-42. (In Russ.)
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