Role of CFT and PCR in diagnosis of Chlamydia psittaci in experimentally infected rabbits

Specific antibodies against сhlamydia weredetectedusingcomplement fixation test and сhlamydiagenome wasdetectedusingpolymerase chain reaction in pregnant rabbits experimentally infected with Chlamydiapsittaci. The infectedrabbitsdevelopeda fever andrespiratory signs andthe infection was confirmed by specificantibodiesagainst сhlamydiadetectedin theirbloodandbyabnormalities in rabbitkindling. Complement fixation testofpairedrabbit serarevealed an increasein thetitersof specificantibodiesagainst сhlamydia, which on Day 7 post infection varied within 1:7.5; on Day 14, mean concentration was 1:40 and by Day 30 mean titer increasedto 1:60. However, when pathological materials from theurogenital tractof theexperimentalanimals weretestedin polymerase chain reaction andin smear microscopy, it was impossibletoconfirm that thereisan etiological linkbetween сhlamydiaandkindlingproblems in experimental animals. At thesametime, molecularandgenetictestsof internalorgans (liver) sampledfromstillborn babyrabbitsrevealedthe сhlamydiagenome, thus, proving сhlamydia involvement into thepathological kindling. Therefore, such a retrospective methodas complement fixation test with a сhlamydia antigen is of high diagnosticvaluefor lifetime сhlamydiadiagnosis.

1. Borovkova L. V., Chelnokova Е. V. The contemporary methods of diagnostics and treating of the sexually transmitted diseases. Medical almanac. 2010; 2 (11): 150–156. EDN: MBFQOB. (in Russ.)

2. Gerasimova V. М., Marchenko V. S., Parfentieva Т. М., Istomina D. N. Comparative characteristics of laboratory methods for the diagnosis of chlamydia. Bulletin of Chelyabinsk State University. Education and Healthcare. 2021; 4 (16): 47–52. DOI: 10.24411/2409-4102-2021-10407. (in Russ.)

3. Guidance on laboratory diagnosis of urogenital infections. Ed. by М. Domeika, А. М. Savicheva, Е. V. Sokolovskiy, R. Ballard, М. Unemo. Saint Petersburg: N-L; 2012. 288 p. (in Russ.)

4. UlyanovaO. V., SaltykovY. V., Zaytsev S. S., Khizhnyakova M. A., Filonova N. N., Subbotina I. A., et al. Laboratory diagnosis of Chlamydia. Guidance. SarRVI; FRCVM; 2019. 29 p. (in Russ.)

5. Harding-Esch E. M., Cousins E. C., Chow S. C., Phillips L. T., Hall C. L., Cooper N., et al. A 30-min nucleic acid amplification point-of-care test for genital Chlamydia trachomatis infection in women: a prospective, multi-centerstudy of diagnostic accuracy. EBioMedicine. 2018; 28: 120–127. DOI: 10.1016/j.ebiom.2017.12.029.

6. Widdice L. E., Hsieh Y. H., Silver B., Barnes M., Barnes P., Gaydos C. A. Performance of the Atlas genetics rapid test for Chlamydia trachomatis and women’s attitudestoward point-of-care testing. Sex. Transm. Dis. 2018; 45 (11): 723–727. DOI: 10.1097/OLQ.0000000000000865.

7. Feodorova V. A., Lyapina А. М., Khizhnyakova М. А., Zaitsev S. S., Saltykov Y. V., Subbotina I. А., et al. Chlamydia of animals and humans. Moscow: Nauka; 2019. 135 p. (in Russ.)

8. Feodorova V. A., Zaitsev S. S., Khizhnyakova M. A., Saltykov Y. V., Evstifeev V. V., Khusainov F. M., et al. Data of de novo genome assembly of the Chlamydia psittacistrain isolated from the livestock inVolga Region, Russian Federation. Data Brief. 2020; 29:105190. DOI: 10.1016/j.dib.2020.105190.

9. Shilova Е. N., Shkuratova I. A., Riaposova M. V., Vyalyh I. V., KozlovaN. A. Effectiveness specific methods of diagnostic chlamydiosis in cattle. Veterinariya. 2014; 4: 21–24. EDN: RZUAYX. (in Russ.)

10. Stothard D. R., Boguslawski G., Jones R. B. Phylogenetic analysis of the Chlamydia trachomatis major outer membrane protein and examination of potential pathogenic determinants. Infect. Immun. 1998; 66 (8): 3618–3625. DOI: 10.1128/IAI.66.8.3618-3625.1998.

11. Ripa T., Nilsson P. A variant of Chlamydia trachomatis with deletion in cryptic plasmid: implicationsfor use of PCR diagnostic tests. Euro Surveill. 2006; 11 (11):E061109.2. DOI: 10.2807/esw.11.45.03076-en.

12. Feodorova V. A., Polyanina T. I., Saltykov Yu. V., Zaytsev S. S., Laskavy V. N., Ulianova O. V., Motin V. L. Detection of Сhlamydia trachomatis in aborted ewes. Veterinariya. 2016; 1: 22–26. EDN: VMFVEV. (in Russ.)

13. Feodorova V. A., Saltykov Y. V., Filonova N. N., Subbotina I. A., Zaitsev S. S., UlianovaO. V., et al. Detection ofthe novel Swedish variant of Chlamydiatrachomatisin cattle. Veterinariya. 2019; 7: 27–31. DOI: 10.30896/0042- 4846.2019.22.7.27-31. (in Russ.)

14. Ripa T., Nilsson P. A. A Chlamydia trachomatis strain with a 377-bp deletion in the cryptic plasmid causing false-negative nucleic acid amplification tests. Sex. Transm. Dis. 2007; 34 (5): 255–256. DOI: 10.1097/OLQ. 0b013e31805ce2b9.

15. Evstifeev V. V., Khusainov F. М., Barbarova L. А., Ravilov А. Z. Usovershenstvovanie komplementsvyazyvayushchego antigena dlya diagnostiki khlamidioza zhivotnykh = Improvement of complement-binding antigen forthe diagnosis of animal Chlamydia. Materialy Vserossiiskoi nauchno-prakticheskoi konferentsii po aktual’nym problemam agropromyshlennogo kompleksa (29–30 oktyabrya 2004 g.) = Proceedings of All-Russian Scientific and Practical Conference on topical problems of the agro-industrial complex (October 29–30, 2004). Kazan: Kazan SAVM; 2004; 33–34. EDN: MYSJIL. (in Russ.)

16. Unemo M., Olcén P., Agné-Stadling I., Feldt A., Jurstrand M., Herrmann B., et al. Experiences with the new genetic variant of Chlamydia trachomatis in Örebro county, Sweden – proportion, characteristics and effective diagnostic solution in an emergent situation. Euro Surveill. 2007; 12 (4):E5-6. DOI: 10.2807/esm.12.04.00699-en.

17. Herrmann B., Törner A., Low N., Klint M., Nilsson A., Velicko I., et al. Emergence and spread of Chlamydia trachomatis variant, Sweden. Emerg. Infect. Dis. 2008; 14 (9): 1462–1465. DOI: 10.3201/eid1409.080153.

18. Feodorova V. A., Polyanina T. I., Konnova S. S., Laskavy V. N. First case of detection of Chlamydia trachomatisin livestock in Russia. In: Proceeding of 5th Congress of EuropeanMicrobiologists (21–25 July 2013, Germany). Leipzig: FEMS; 2013; 237.

19. Feodorova V. A., Zaitsev S. S., Saltykov Y. V., Sultanakhmedov E. S., Bakulev A. L., Ulyanov S. S., Motin V. L. An asymptomatic patient with fatal infertility carried a Swedish strain of Chlamydia trachomatis with additional deletion in the plasmid orf1 that belonged to a different MLST sequence type. Microorganisms. 2019; 7 (7):187. DOI: 10.3390/microorganisms7070187.

20. Evstifeev V. V., Khusainov F. M., Yakovlev S. I., Khusainova G. I., Ivanova S. V. Results of clinical trials of a universal vaccine against Chlamydia in farm animals. Nauchnye osnovy proizvodstva i obespecheniya kachestva biologicheskikh preparatov: materialy Mezhdunarodnoi nauchno-prakticheskoi konferentsii, posvyashchennoi 100-letiyu Armavirskoi biofabriki (20–21 avgusta 2021 g.) = Scientific basisfor production and quality assurance of biological preparations: proceedings of the international research-to-practice conference dedicated to the 100th anniversary of the Armavir Biofactory (20–21 August 2021). Мoscow: 2021; 244–251. DOI: 10.47804/978-5-89904-0290_2021_244. (in Russ.)