Bovine leukemia virus occurrence in Dagestan

Results of diagnostic tests for bovine leukosis carried out in the Dagestan Republic in 2022 (as of October 1, 2022) showing bovine leukemia virus (BLV) occurrence are presented in the paper for the whole Republic and for each municipality separately. In total, 632,454 susceptible animals were serologically tested with immunodiffusion assay; 3,573 reactor animals (0.6% of tested animals) were detected. Proportion of infected animals was as follows: 0.5% – in administrative raions, 1.6% – in urban districts and 1.1% – in distant pasture zones. Percentage of infected cattle varied from 0.01 to 4.9%. No hematological examinations for bovine leukosis were carried out because seropositive animals were timely culled. In the breeding sector, the proportion of bovine leukemia virus carriers was averagely 2.8%. Ninety-five bovine leukosis-affected localities were reported in the region as of 1 January 2022. Eighty-one new BLV-infected localities had been identified and bovine leukosis had been eliminated in 18 localities for 9 months of 2022. Totally, 158 localities were officially declared affected in 2022 (as of October 1, 2022): 36 agricultural holdings (including 5 breeding holdings), 18 small-scale farms and 104 backyard farms. The largest number of bovine leukosis-affected localities was registered in the Kizlyarsky (18), Tarumovsky (17), Babayurtovsky (16), Gunibsky (15), Tlyaratinsky (10) Raions and in the city of Makhachkala (9). One disease-affected locality was reported in each of the Bezhtinsky, Buynaksky, Derbentsky, Kazbekovsky, Kayakentsky, Kizilyurtovsky, Khasavyurtovsky Raions and towns of Khasavyurt and Yuzhno-Sukhokumsk. Two disease-affected localities were reported in each of the Rutulsky, Untsukulsky Raions, three disease-affected localities were reported in each of the Gergebilsky, Laksky, Novolaksky, Tsumadinsky Raions. Four disease-affected localities were reported in the Sergokalinsky Raion, five disease-affected localities were reported in the Charodinsky Raion, six disease-affected localities were reported in each of the Akhvakhsky, Dakhadaevsky, Karabudakhkentsky Raions, seven disease-affected localities were reported in each of the Botlikhsky, Kumtorkalinsky and Shamilsky Raions. Comparative analysis of serological and molecular genetic methods used for bovine leukosis diagnosis demonstrated the advantage of enzyme-linked immunosorbent assay and polymerase chain reaction as compared to immunodiffusion assay used in veterinary practice.

1. Gulyukin M. I., Gulyukin A. M., Donchenko A. S., Donchenko N. A., Barsukov Yu. I., Loginov S. I., et al. Analysis of the episootic situation of cattle leukemia in the Siberian Federal District. Siberian Herald of Agricultural Science. 2021; 51 (4): 67–75. DOI: 10.26898/0370-8799-2021-4-8. (in Russ.)

2. Zyuzgina S. V., ZinovievaO. E., Nurlygayanova G. A. Analysis of laboratory diagnosis of bovine leukemia virus in the North Caucasus Federal District from 2019 to 2021. Mining agriculture. 2022; 3: 72–75. DOI: 10.25691/GSH.2022.3.017. (in Russ.)

3. Skhatum A. K., Basova N. Yu., Staroselov M. A., Pachina V. V., Tikhonov S. V. Epizootic situation on bovine leucose in farms of Krasnodar region. Veterinaria Kubani. 2019; 3: 10–13. DOI: 10.33861/2071-8020-2019-3-10-13. (in Russ.)

4. Gulykin M. I., Barabanov I. I, Ivanova L. A., Stepanova T. V., Kozireva N. G., Simonian G. A., et al. Monitoring of epidemiologic situation with Bovine Leukemia in production and breeding herds of Russian Federation in 2014–2015. Veterinaria i kormlenie. 2016; 4: 5–41. EDN: WFIZOZ. (in Russ.)

5. Gulyukin M. I., Zaberezhny A. D., Yurov K. P., Shabeykin A. A., Barabanov I. I., Stepanova T. V., Lopunov S. V. Scientifically sound model of antiepizootic measuresin the bovine leukemia. Veterinaria i kormlenie. 2018; 1: 4–7. DOI: 10.30917/ATT-VK-1814-9588-2018-1-1. (in Russ.)

6. Tazayan A. N., Tambiev T. S., Vasiliev A. V. Monitoring of the epizootic situation with cattle leukosis in the Rostov Oblast. International Research Journal. 2022; 8 (122). DOI: 10.23670/IRJ.2022.122.51. (in Russ.)

7. Karaulov A. K., Varkentin А. V., Petrova О. N., Tatsenko Е. Е., Semenova Е. А., Scherbinin S. V., et al. Epizootic situation in the Russian Federation in 2021. FGBI “ARRIAH”. Available at: https://fsvps.gov.ru/sites/default/files/files/iac/2022/2021_31_12_godovoy_otchet.pdf. (in Russ.)

8. Budulov N. R., Ustarkhanov P. D., Salikhov Yu. S., Mustafayev A. R. Epizooticheskaya obstanovka po leikozu krupnogo rogatogo skota v sel’khozpredpriyatiyakh Dagestana = Epizootic situation for bovine leukosis in agricultural holdings of Dagestan. Vestnik veterinarii. 2004; 3 (30): 7–12. EDN: JUSXGD. (in Russ.)

9. Budulov N. R., Nuratinov R. A. Epizootologicheskii monitoring leikoza i tuberkuleza krupnogo rogatogo skota v khozyaistvakh Respubliki Dagestan = Epizootological monitoring of bovine leukosis and tuberculosis in holdings located in the Republic of Dagestan. Veterinarnaya patologiya. 2007; 2 (21): 123–127. EDN: OEZJNT. (in Russ.)

10. Kabardiev S. Sh., Budulov N. R., Gaydarbekova H. M., Ragimova T. T. Epizooticheskaya situatsiya po leikozu krupnogo rogatogo skota v plemennykh khozyaistvakh Dagestana = Epizootic situation for bovine leukosis in breeding holdings in Dagestan. Veterinarnaya patologiya. 2008; 2 (25): 67–68. EDN: OEDSZF. (in Russ.)

11. Budulov N. R., Shikhragimov E. M., Musaeva M. N., Salikhov Yu. S., Gaydarbekova H. M. Leucosis of cattle in the Cis-Caspian Region of Russian Federation. Vestnik veterinarii. 2012; 3 (62): 45–51. EDN: PAVARL. (in Russ.)

12. Budulov N. R., Salikhov Yu. S., Shikhragimov E. M., Musaeva M. N., Gaydarbekova H. M. Epizootic situation concerning bovine leukemia in the Dagestan Republic during the last three years. Vestnik veterinarii. 2016; 2 (77): 62–65. EDN: VZRSOJ. (in Russ.)

13. Budulov N. R., Shikhragimov E. M., Salikhov Yu. S., Musaeva M. N., Gaydarbekova H. M. Dynamics of the spread of leukemia of cattle in Dagestan Republic. Veterinaria i kormlenie. 2017; 5: 23–25. EDN: ZOFMSF. (in Russ.)

14. Budulov N. R., Aliev A. Yu. Distribution and control measures with cattle leukemia in Dagestan Republic. Veterinariya. 2021; 6: 15–20. DOI: 10.30896/0042-4846/2021.24.6.15-20. (in Russ.)

15. Budulov N. R., Yusupov O. Yu., Salikhov Yu. S., Shikhragimov E. M. Monitoring of cattle leukosis in the breeding farms of the Dagestan Republic. Veterinary pathology. 2020; 2 (72): 25–30. DOI: 10.25690/VETPAT.2020.72.2.007. (in Russ.)

16. Budulov N. R. Objective epizootic situation on cattle leukemia in Dagestan. Veterinaria i kormlenie. 2021; 4: 15–18. DOI: 10.30917/ATT-VK-1814-9588-2021-4-4. (in Russ.)

17. Ponomareva I. S., Sycheva M. V., Polyakov M. A., Nurgalieva R. M., KartashovaO. L. Effektivnost’ diagnostiki leikoza krupnogo rogatogo skota metodami RID, IFA i PTsR v khozyaistvakh Orenburgskoi Oblasti = Effectiveness of bovine leukosis diagnosis with AGID, ELISA and PCR in holdings located in the Orenburg Oblast. Modern High Technologies. 2010; 9: 134. EDN: NAWUPJ. (in Russ.)

18. Donnik I., Ponomareva O., Chernykh O., Lysenko A., Mikailov M., Gunashev Sh., et al. Improving diagnostic and eliminating techniques of bovine leukemia in the Russian Federation. JPRI. 2021; 33 (60B): 3078–3084. DOI: 10.9734/jpri/2021/v33i60B34980.

19. Mohammadabadi M. R., Soflaei M., Mostafavi H., Honarmand M. Using PCR for early diagnosis of bovine leukemia virus infection in some native cattle. Genet. Mol. Res. 2011; 10 (4): 2658–2663. DOI: 10.4238/2011.October.27.2.

20. Petropavlovskiy M. V., BezborodovaN. A., Romanova A. S., Lysov A. V., Kozhukhovskaya V. V. Experience in the use of polymerase chain reaction in the diagnosis of bovine leukemia virus and its effectiveness at different stages of health activities. Agrarian Bulletin of the Urals. 2019; 12 (191): 52–59. DOI: 10.32417/1997-4868-2019-191-12-52-59. (in Russ.)

21. Loginov S. I. Analysis of the effectiveness of the use of enzyme-linked immunosorbent assay for the diagnosis of leukemia in cattle during health-improving activities. Bulletin of NSAU (Novosibirsk State Agrarian University). 2020; (4): 95–102. DOI: 10.31677/2072-6724-2020-57-4-95-102. (in Russ.)

22. Saepulloh M., Sendow I. Efektivitas metode PCR dan AGID dalam mendeteksi penyakit Enzootic Bovine Leucosis di Indonesia = Effectivity of PCR and AGID methods to detect of enzootic bovine leucosis in Indonesia. JITV. 2015; 20 (1): 71–78. DOI: 10.10.14334/jitv.v20il.1120. (in Indonesian)

23. Konopatkin А. А., Bakulov I. А., Nuikin Ya. V., Artemov B. Т., Bessarabov B. F., Kadymova R. А., et al. Epizootology and infectious livestock diseases: study guide. Ed. by A. A. Konopatkin. Moscow: Kolos; 1984. 544 p. (in Russ.)

24. Ponomareva О. I., Chernykh О. Yu., Lysenko А. А., Dmitriev А. F., Belyaev V. А., Budulov N. R., et al. Methodical guidelines for bovine leukosis diagnosis and shortening the disease elimination period in the disease-affected holdingsin the Republic of Dagestan. Makhachkala; 2021; 62 p. (in Russ.)

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