RESULTS OF EXPERIMENTAL INFECTION OF TURKEYS WITH A/DUCK/ALTAI/469/14 H5N1 STRAIN OF AVIAN INFLUENZA VIRUS

The data on experimental infection of 6-week-old Big-6 cross turkeys with an epidemic A/duck/Altai/469/14 H5N1 clade 2.3.2.1c strain of avian influenza virus are presented. The characteristics of the infection process in birds inoculated intranasally at a dose of 5.0 lg EID50/0.5 cm3 are described with an indication of the incubation period and the mean time of death. The pathomorphological changes at the tissue and cellular level are shown based on histological and immunohistochemical studies of fragments of respiratory, digestive, cardiovascular, nervous, excretory, lymphoid and muscular systems of experimental birds. The testing was carried out using paired preparations of paraffin-embedded tissue sections from experimentally infected and healthy turkeys. One sample was subjected to histological staining using hematoxylin and eosin dyes, and its duplicate was subjected to immunohistochemical assay using a preparation of polyclonal antibodies as primary antibodies against the ribonucleoprotein of avian influenza virus. The results of histological and immunohistochemical studies are photodocumented and presented in the paper. Inflammatory and necrotic lesions of varying severity are detected in the preparations of the trachea, lung, muscular stomach, glandular stomach, small intestine, large intestine, pancreas, brain, cerebellum, heart, kidneys, liver and spleen of turkeys. Immunohistochemical analysis showed the greatest distribution of the influenza virus antigen in the cerebral endothelium, cerebellar Purkinje neurons, acinar cells of the pancreas and in myocardiocytes of the heart. In the course of the experiment it was established that A/duck/Altai/469/14 H5N1 caused a generalized form of infection in turkeys with clinical and pathologic lesions characteristic of highly pathogenic avian influenza.

avian influenza, H5N1, turkey, experimental infection, histology, immunohistochemistry

1. Ganenko I., Belaya A. Turkey production will decrease by 2% in 2017. Agroinvestor. Markets. News. URL: http://www.agroinvestor.ru/markets/news/28938-proizvodstvo-indeyki-po-itogam2017-goda-sokratitsy/ (in Russian).

2. Study of pathological process induced by А/duck/Altai/469/14 H5N1 isolate of the avian influenza virus. V. Yu. Sosipatorova, D. А. Altunin, М. А. Tzivanyuk, И. А. Chvala. Veterinary Science Today. 2016; 1 (16): 51–54 (in Russian).

3. Pathomorphological changes in chicken experimentally infected with A/H5N1 avian influenza virus. I. V. Bakhchin, I. А. Chvala, М. А. Volkova [et al.]. Vestnik veterinarii. 2014; 1 (68): 47–51 (in Russian).

4. Lethality parameters during experimental and natural infection induced by highly pathogenic avian influenza Н5N1 virus. А. V. Zaikovskaya, S. А. Leonov, Yu. G. Yushkov [et al.]. Bjulleten’ VSNC SO RAMN. 2012: 5 (87): 224–227 (in Russian). 5. Animal Health in the Word. Avian Influenza Portal. OIE. URL:http://www.oie.int/en/animal-health-in-the-world/updateon-avian-influenza/.

5. Enhanced virulence of clade 2.3.2.1 highly pathogenic avian influenza A H5N1 viruses in ferrets. M. B. Pearcea, C. Pappasa, K. M. Gustina [et al.]. Virology. 2017; 502: 114–122; doi: 10.1016/j.virol.2016.12.024.

6. Highly pathogenic avian influenza H5N1 clade 2.3.2.1 and clade 2.3.4 viruses do not induce a clade-specific phenotype in mallard ducks. M. Ducatez, S. Sonnberg, J. C. Crumpton [et al.]. J. Gen. Virol. 2017; 98: 1232–1244; doi: 10.1099/jgv.0.000806.

7. Infection dynamics of highly pathogenic avian influenza and virulent avian paramyxovirus type 1 viruses in chickens, turkeys and ducks. E. W. Aldous, J. M. Seekings, A. McNally [et al.]. Avian Pathol. 2010; 39 (4): P. 265–273; doi: 10.1080/03079457.2010.492825.

8. Pathobiology and transmission of highly and low pathogenic avian influenza viruses in European quail (Coturnix c. coturnix). K. Bertran, R. Dolz, N. Busquets [et al.]. Vet. Res. 2013; 44 (1): 23; doi: 10.1186/1297-9716-44-23.

9. Pathogenicity and transmission of H5N1 avian influenza viruses in different birds. R. Yuan, J. Cui, S. Zhang [et al.]. Vet. Microbiol. 2014; 168 (1): 50–59; doi: 10.1016/j.vetmic.2013.10.013.

10. Pathological findings of highly pathogenic avian influenza virus A/Duck/Vietnam/12/2005 (H5N1) in Turkeys. M. J. Mehrabanpour, H. Dadras, A. Khodakaram-Tafti [et al.]. Intern. J. Poultry Sci. 2007; 6 (9): 679–683; doi: 10.3923/ijps.2007.679.683.

11. Phylogenetic and pathogenic analyses of three H5N1 avian influenza viruses (clade 2.3.2.1) isolated from wild birds in Northeast China. Z. Fan, Y. Ci, L. Liu [et al.]. Infect. Genet. Evol. 2015; 29: 138–145; doi: 10.1016/j.meegid.2014.11.020.

12. Protective efficacy of H5 inactivated vaccines in meat turkey poults after challenge with Egyptian variant highly pathogenic avian influenza H5N1 virus. W. H. Kilany, E. M. Abdelwhab, A. S. Arafa [et al.]. Vet. Microbiol. 2010; 150 (1–2): 28–34; doi: 10.1016/j.vetmic.2010.12.016.