EFFECTIVENESS OF SEROLOGICAL TESTS FOR DIAGNOSTICS OF BOVINE EPHEMERAL FEVER

Review of publications devoted to spread of bovine ephemeral fever (BEF) in the world is presented; epizootic aspect of this problem is highlighted. The disease is not registered in Americas, Island, New Zealand and Europe. African continent, Australia and Asian countries including those neighboring the Russian Federation are considered BEF infected, that is why there is a risk of BEF introduction into our country. Serological tests for BEFV antibodies in the Russian Federation were performed for the first time. In total 13,911 samples, collected in 2008-2010 on farms in 26 regions of the Russian Federation were tested. Testing of cattle sera using prolonged complement fixation test revealed 333 BEFV seropositive samples, submitted from 9 regions in 2008, percentage by region was 2.6-25.9%, average percentage was 9.7% out of the total number of samples tested during the abovementioned period. Sera specific activity was determined when they were used non-diluted or diluted at 1:10-1:20. Sera with positive reactions in prolonged CFT showed specific activity in ELISA at 1:10-1:1,280 dilutions, but if diffusion precipitation test with non-diluted sera was used antibodies were detected only in few samples due to insufficient sensitivity. Test results proved the possibility of serological monitoring using prolonged CFT and ELISA. Due to insufficient sensitivity of diffusion precipitation test, its use for this purpose is not feasible.

эфемерная лихорадка КРС, РДСК, ИФА, РДП, антитела, диагностика, bovine ephemeral fever, prolonged CFT, ELISA, diffusion precipitation test, antibodies, diagnostics

1. Курченко Ф. П. Эфемерная лихорадка крупного рогатого скота (обзор иностранной литературы) // Ветеринария. -1985. - № 6. - С. 67-69.

2. Макаров В. В. Современные данные об эфемерной лихорадке крупного рогатого скота // Сельское хозяйство за рубежом. - 1974. - № 11. - С. 34-36.

3. Мирзаев Д. Профилактика и меры борьбы с эфемерной лихорадкой // Сельское хозяйство Таджикистана. - 1984. -№ 12. - С. 46.

4. Мониторинг распространения и предотвращения особо опасных болезней животных: сб. материалов конф., Самарканд, 26-27 окт. 2004 г. - Самарканд, 2004. - С. 107-110.

5. Экспериментальные исследования по изучению клиники эфемерной лихорадки крупного рогатого скота и получению диагностических препаратов / В. И. Диев [и др.] // Вирусные и микробные болезни животных: сб. науч. тр. - Владимир, 1995. - С. 128-131.

6. A large-scale outbreak of bovine ephemeral fever in Turkey, 2012 / S. Tonbak [et al.] // J. Vet. Med. Sci. - 2013. - Vol. 75, No. 11. -P. 1511-1514.

7. Application of a real-time polymerase chain reaction assay to the diagnosis of bovine ephemeral fever during an outbreak in New South Wales and northern Victoria in 2009-10 / D. S. Finlaison [et al.] // Aust. Vet. J. - 2014. - Vol. 92, No. 1-2. - P. 24-27.

8. Circulation of bovine ephemeral fever in the Middle East -strong evidence for transmission by winds and animal transport / O. Aziz-Boaron [et al.] // Vet. Microbiol. - 2012. - Vol. 158, No. 3-4. -P. 300-307.

9. Epidemiological analysis of bovine ephemeral fever in 2012-2013 in the subtropical islands of Japan / Y. Hayama [et al.] // BMC Vet. Res. - 2016. - URL: https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-016-0673-0 (дата обращения: 03.08.16).

10. Epidemiological investigation of bovine ephemeral fever outbreaks in Israel / I. Yeruham [et al.] // Vet. Med. Int. - 2010. -URL: http://www.hindawi.com/journals/vmi/2010/290541/ (дата обращения: 03.08.16).

11. Epizootics of bowine ephemeral fewer on dairy farms in Saudi Arabia / M. A. Farag [et al.] // Rev. Sci. Tech. OIE. - 1998. -Vol. 17, No. 3. - P. 713-722.

12. Finlaison D. S., Read A. J., Kirkland P. D. An epizootic of bovine ephemeral fever in New South Wales in 2008 associated with long-distance dispersal of vectors // Aust. Vet. J. - 2010. - Vol. 88, No. 8. - P. 301-306.

13. Large-scale serological survey of bovine ephemeral fever in China / Z. Li [et al.] // Vet. Microbiol. - 2015. - Vol. 176, No. 1-2. -P. 155-160.

14. Molecular epidemiology of Bovine ephemeral fever virus in cattle and buffaloes in Iran / H. Momtaz [et al.] // Revue Méd. Vét. - 2012. - Vol. 163, No. 8-9. - P. 415-418.

15. Occurrence of bovine ephemeral fever in Okinawa Prefecture, Japan, in 2012 and development of a reverse-transcription polymerase chain reaction assay to detect bovine ephemeral fever virus gene / T. Niwa [et al.] // J. Vet. Med. Sci. - 2015. - Vol. 77, No. 4. - P. 455-460.

16. Preliminary studies of the complement fixation test to confirm the diagnosis of bovine ephemeral fever / Bai Wenbin [et al.] // Aust. J. Biol. Sci. - 1987. - Vol. 40, No. 2. - P. 137-141.

17. Relationships of bovine ephemeral fever epizootics to population immunity and virus variation / L.-J. Ting [et al.] // Vet. Microbiol. - 2014. -Vol. 173, No. 3-4. - P. 241-248.

18. Resurgence of bovine ephemeral fever in mainland Japan in 2015 after a 23-year absence / Y. Hirashima [et al.] // J. Vet. Med. Sci. - 2017. - Vol. 79, No. 5. - P. 904-911.

19. Sero-survey on Aino, Akabane, Chuzan, bovine ephemeral fever and Japanese encephalitis virus of cattle and swine in Korea / S. In Lim [et al.] // J. Vet. Sci. - 2007. - Vol. 8, No. 1. - P. 45-49.

20. The follow up study after massive outbreak of Akabane and bovine ephemeral fever viruses in Korea / D. K. Yang [et al.] // Korean J. Vet. Serv. - 2013. - Vol. 36, No. 3. - P. 151-155.

21. The geographical distribution and first molecular analysis of Culicoides Latreille (Diptera: Ceratopogonidae) species in the Southern and Southeastern Turkey during the 2012 outbreak of bovine ephemeral fever / B. Dik [et al.] // Parasitol. Res. -2014. - Vol. 113, No. 11. - P. 4225-4232.

22. The history of bluetongue, Akabane and ephemeral fever viruses and their vectors in Australia 1975-1999 / T. D. St. George [et al.]. - Canberra, Australia, 2001. - 101 p. - URL: https://espace.library.uq.edu.au/view/UQ:290721/TSG_2_34.pdf (дата обращения: 05.08.16).

23. Zheng F., Qiu C. Phylogenetic relationships of the glycoprotein gene of bovine ephemeral fever virus isolated from mainland China, Taiwan, Japan, Turkey, Israel and Australia // Virol. J. - 2012. - Vol. 9. - URL: http://virologyj.biomedcentral.com/articles/10.1186/1743-422X-9-268 (дата обращения: 05.08.16).