Identification of Haemophilus parasuis using MALDI-TOF mass spectrometry
Abstract
The paper covers results of MALDI-TOF mass spectrometry used to rapidly identify Haemophilus parasuis field isolates. ClinProTools software showed 15 peaks in the protein spectrum with 100% identification capability differentiating 6 Pasteurellaceae species. Peak corresponding to mass of 8407.92 Da (p<0.05) is unique for Haemophilus parasuis. The updated database was successfully used for identification of 8 Haemophilus parasuis isolates. No false-positive results were obtained when the method specificity was tested using 9 bacterial species of Pasteurellaceae family.
About the Authors
V. I. Pavelko
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
Yu. Yu. Babin
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
A. V. Piskunov
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
A. V. Sprygin
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
O. V. Pruntova
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
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Review
For citations:
Pavelko V.I., Babin Yu.Yu., Piskunov A.V., Sprygin A.V., Pruntova O.V. Identification of Haemophilus parasuis using MALDI-TOF mass spectrometry. Veterinary Science Today. 2016;(2):61-65. (In Russ.)
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