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Preparation of specific components of Elisa test-system for detection of Schmallenberg desease virus antigens

Abstract

Preparation of purified and concentrated antigens of Schmallenberg disease virus as well as specific hyperimmune sera in laboratory animals used for the development of indirect sandwich enzyme-linked immunosorbent assay (ELISA) is described in the paper.

About the Authors

O. G. Gubenko
FGBI "ARRIAH", Vladimir
Russian Federation


O. P. Bjadovskaya
FGBI "ARRIAH", Vladimir
Russian Federation


S. V. Kononova
FGBI "ARRIAH", Vladimir
Russian Federation


Yu. Yu. Babin
FGBI "ARRIAH", Vladimir
Russian Federation


N. A. Soloukhina
Vladimir University at the FGBI «ARRIAH»
Russian Federation


A. V. Kononov
FGBI "ARRIAH", Vladimir
Russian Federation


References

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2. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophageT4 // Nature. - 1970. - Vol. 227. - P. 680-685.

3. Nucleocapsid protein structures from orthobunyaviruses reveal insight into ribonucleoprotein architecture and RNA polymerization / A. Ariza, S.J. Tanner, C.T. Walter [etal.] // Nucleic Acids Res. - 2013. - Vol. 41. - P. 5912 - 5926.

4. Organ distribution of Schmallenberg virus RNA in malformednewborns / S. Bilk, C. Schulze, M. Fischer, M. Beer // Vet. Microbiol. - 2012. - Vol. 159. - P. 236-238.

5. Zhang Y., Wu S., Wang J. Expression and purification of the nucleocapsid protein of Schmallenberg virus, and preparation and characterization of a monoclonal antibody against this protein // Protein Exp. Purifie. - 2013.- Vol. 92, - P. 1-8.


Review

For citations:


Gubenko O.G., Bjadovskaya O.P., Kononova S.V., Babin Yu.Yu., Soloukhina N.A., Kononov A.V. Preparation of specific components of Elisa test-system for detection of Schmallenberg desease virus antigens. Veterinary Science Today. 2016;(2):52-55. (In Russ.)

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ISSN 2304-196X (Print)
ISSN 2658-6959 (Online)