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Comparative evaluation of media used for detection of Salmonella spp

Abstract

The paper demonstrates evaluation results for productive and selective capacities of domestic and imported chromogenic nutrient media (Bismuth sulphite agar and xylose lysine deoxycholate agar (XLD agar)). The productivity ratio of imported and domestic XLD agar amounted to over 0.58 and selectivity ratio - to less than 2 Ig. The productivity ratio of imported Bismuth sulfate agar was higher than the productivity of the domestic one and amounted to over 0.19. The selective properties of the both media are similar and amount to 5 Ig. Based on the evidence found, imported Bismuth sulfate agar and domestic or imported XLD agar can be recommended for use in the experiments for detection of Salmonella spp in food products.

About the Authors

G. S. Skitovich
FGBI "ARRIAH", Vladimir
Russian Federation


K. V. Serova
FGBI "ARRIAH", Vladimir
Russian Federation


N. B. Shadrova
FGBI "ARRIAH", Vladimir
Russian Federation


O. V. Pruntova
FGBI "ARRIAH", Vladimir
Russian Federation


References

1. Ашмарин И.П., Васильев H.H., Амбросов В.А. Быстрые методы статистической обработки и планирование экспериментов. - Л.: Изд-во Ленингр. ун-та, 1974. -76 с.

2. Бхуниа А.К. Патогенные микроорганизмы пищевых продуктов. - СПб.: Профессия, 2014. - 344 с.

3. ГОСТ ISO 11133-2-2011. Микробиология пищевых продуктов и кормов для животных. Руководящие указания по приготовлению и производству культуральных сред. Часть 2. Практические руководящие указания по эксплуатационным испытаниям культуральных сред. - М.: Стандартинформ, 2013. - 32 с.

4. Микробиологический анализ мяса, мяса птицы и яйцепродуктов / под ред. Дж.К. Мид; пер. с англ. - СПб.: Профессия, 2008. - 384 с.

5. BBL™XLD Agar. Quality control procedures. - Becton, Dickinson and Company, Sparks, MD, 2007. - L007426, Rev. 09. - URL: http://www.bd.com/ds/technicalCenter/inserts/L007426%2809%29%280907%29.pdf.

6. Detection of Salmonella serovars from clinical samples by enrichment broth cultivation-PCR procedure / G.G. Stone [et al.] // J. Clin. Microbiol. - 1994. - Vol. 32, № 7, - P. 1742-1749.

7. Manafi M. New developments in chromogenic and fluorogenic culture media // Int. J. Food Microbiol. - 2000, - Vol. 60, № 2-3. - P. 205-218.

8. Park S.-H., Ryu S. Kang D.-H. Development of an improved selective and differential medium for isolation of Salmonella spp. // J. Clin. Microbiol. - 2012. - Vol. 50, № 10, - P. 3222-3226.

9. Van der Zee H. Media for isolation of Salmonella H Progress in Industrial Microbiology. Handbook of Culture media for Food Microbiology / ed. J.E.L. Corry, G.D.W. Curtis, R.M. Baird. - Amsterdam, Netherlands: Elsevier, 2003, - Vol. 37, - P. 195-208.


Review

For citations:


Skitovich G.S., Serova K.V., Shadrova N.B., Pruntova O.V. Comparative evaluation of media used for detection of Salmonella spp. Veterinary Science Today. 2016;(2):39-45. (In Russ.)

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ISSN 2304-196X (Print)
ISSN 2658-6959 (Online)