Proteomic properties of Salmonella isolates

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Аннотация

The paper presents results of Salmonella of studying Salmonella isolate properties by MALDI-TOF method using MALDI Autoflex III mass-spectrometer. Microorganism identification was performed using direct loading method followed by comparison of mass spectra with the database set in the apparatus. Peaks typical of many Enterobacteriaceae representatives and mass to charge ratio (m/z), characterizing Salmonella were determined.

Об авторах

N. Shadrova
FGBI «ARRIAH
Россия


O. Pruntova
FGBI «ARRIAH
Россия


G. Skitovich
FGBI «ARRIAH
Россия


Список литературы

1. Bhunia A.K. Pathogenic microorganisms in food products. - Saint-Petersburg: Professia, 2014. - 344 p.

2. Time-of-light MALDI mass-spectrometry for microorganism identification / Ye.A. Demidov, K.V. Starostin, V.M. Popik, S.Ye. Peltek // Vavilov Journal of Genetics and Breeding. - 2013. - Vol. 17, № 4/1. - P. 758-764.

3. Anhalt J.P., Fenselau C. Identification of bacteria using mass spectrometry // Analytical Chemistry. - 1975. -Vol. 47.-P. 219-225.

4. Ben L.M., van Baar Characterisation of bacteria by matrix-assisted laser desorption/ionization and electrospray mass spectrometry // FEMS Microbiol. Rev. - 2000. -Vol. 24, № 2.-P. 193-219.

5. Dieckmann R., Malorny В. Rapid screening of epide-miologically important Salmonella enterica subsp. enterica serovars using Whole-Cell MALDI-TOF mass spectrometry // Appl. Environ. Microbiol. - 2011. - Vol. 77, № 12.-P. 4136-4146.

6. MALDI Biotyper User Manual 2008, Version 2.0. SRI; Bruker Daltonic Inc.: Bremen, Germany, 2008. -130 p.

7. Matrix-assisted ultraviolet-laser desorption of nonvolatile compounds / M. Karas, D. Bachmann, U. Bahr, F. Hillencamp// Int. J. Mass Spectrom. Ion. Process. -1987. -Vol. 78.-P. 53-68.

8. McWhorter A., Haddock R, Nocon F. Trabulsiella guamensis, a new genus new species of the family Enterobacteriaceae that resembles Salmonella subgroups

9. and 5 /J. Clin. Microbiol. - 1991. - Vol. 29. - P. 1480-1485.

10. Protein and polymer analyses up to m/z 100 000 by laser ionization time-of-flight mass spectrometry/K. Tanaka, H.Waki.Y. Ido [etal.]//Rapid Commun. Mass Spectrom. -1988.-Vol. 2, № 8.-P. 151-153.

11. Rapid identification and typing of listeria species by matrix-assisted laser desorption ionization-time of flight mass spectrometry / S.B. Barbuddhe.T. Maier, G. Schwarz [et al.] // Appl. Environ. Microbiol. - 2008. - Vol. 74, № 17. - P. 5402-5407.

12. Ryzhov V., Fenselau C. Characterization of the protein subset desorbed by MALDI from whole bacterial cells // Analyt. Chem. - 2001. - Vol. 73, № 4. -P. 746-750.

13. Whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of bacteria cultured in liquid media/N. Zhou, N. Wang, B.Xu [etal.]//Science China Life Sciences.-201 l.-Vol. 54, № 1. - P. 48-53.


Дополнительные файлы

Для цитирования: ., ., . . Ветеринария сегодня. 2016;(2):35-38.

For citation: Shadrova N.B., Pruntova O.V., Skitovich G.S. Proteomic properties of Salmonella isolates. Veterinary Science Today. 2016;(2):35-38. (In Russ.)

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