Validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich Elisa
Abstract
The paper describes the process of the validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich ELISA for FMD types A, 0 and Asial. The results were analyzed and the following main validation characteristics were determined: sensitivity, specificity, accuracy, consistency, predictability of positive and negative results, reproducibility and intermediate precision under the conditions of repeatability and reproducibility. The results of the ELISA procedure validation were in compliance with the acceptability criteria.
About the Authors
N. N. Lugovskaya
ФГБУ «ВНИИЗЖ», г. Владимир
Russian Federation
Russian Federation
Ye. N. Kalinina
ФГБУ «ВНИИЗЖ», г. Владимир
Russian Federation
Russian Federation
K. S. Maikova
ФГБУ «ВНИИЗЖ», г. Владимир
Russian Federation
Russian Federation
O. V. Vorobyova
ФГБУ «ВНИИЗЖ», г. Владимир
Russian Federation
Russian Federation
G. M. Goryacheva
ФГБУ «ВНИИЗЖ», г. Владимир
Russian Federation
Russian Federation
T. K. Mayorova
ФГБУ «ВНИИЗЖ», г. Владимир
Russian Federation
Russian Federation
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Review
For citations:
Lugovskaya N.N., Kalinina Ye.N., Maikova K.S., Vorobyova O.V., Goryacheva G.M., Mayorova T.K. Validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich Elisa. Veterinary Science Today. 2015;(3):22-29. (In Russ.)
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