Cloning and expression of African swine fever virus K205R and B602L genes inf. COLI
Аннотация
A molecular cloning of African swine fever virus k205r and b602l genes in £ coli was carried out. The expression and purification conditions ensuring high yield of recombinant proteins were optimized. Dissolved recombinant proteins were purified by metal-chelate affinity chromatography using Ni-NTA-agarose («Qiagen»). Recombinant antigens are biologically safe, easier to prepare and ensure higher ELISA specificity. The purified protein yield from 100 ml off. coli culture was 1,5 mg for pK205R and 2 mg for pB602L.
Об авторах
A. Scherbakov
FGBI "ARRIAH", Vladimir
Россия
Россия
A. Yakovleva
FGBI "ARRIAH", Vladimir
Россия
Россия
A. Timina
FGBI "ARRIAH", Vladimir
Россия
Россия
M. Yakupov
FGBI "ARRIAH", Vladimir
Россия
Россия
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Рецензия
Для цитирования:
, , , . Ветеринария сегодня. 2015;(2):31-35.
For citation:
Scherbakov A.V., Yakovleva A.S., Timina A.M., Yakupov M.R. Cloning and expression of African swine fever virus K205R and B602L genes inf. COLI. Veterinary Science Today. 2015;(2):31-35. (In Russ.)
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