Development of polimeraze chain reaction based test system for Eperythrozoon suis detection

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Аннотация

A PCR and electrophoresis based tool was developed for Eperythrozoon suis detection. During 2004-2013, tests for Eperythrozoon suis detection were performed using the developed tool. The agent was detected in whole blood samples collected from replacement gilts and sows.

Об авторах

M. Biryuchenkova
FGBI «ARRIAH», Vladimir
Россия


A. Timina
timina@arriah.ru
Россия


Список литературы

1. Development of a diagnostic PCR assay based on novel DNA sequences for the detection of Mycoplasma suis (Eperythrozoon suis) in porcine blood / L. E. Hoelzle [et al.] //Vet. Microbiol. - 2003. - Vol. 93. - P. 185-196.

2. Exploratory study of Mycoplasma suis (Eperythrozoon suis) on four commercial pig farms in southern Brazil/ A. M. Guimaraes [etal.] //Vet. Rec. - 2007. - Vol. 160, № 2,- P. 50-53.

3. First LightCycler real-time PCR assay for the quantitative detection of Mycoplasma suis in clinical samples / L. E. Hoelzle [et al.] // J. Microbiol. Methods. - 2007.- Vol. 70,- P. 346-354.

4. Hoelzle L. E. Haemotrophic mycoplasmas: recent advances in Mycoplasma suis//Vet. Microbiol.- 2008.- Vol. 130, - P. 215-226.

5. Mycoplasma suis infection results endothelial cell damage and activation: new insight into the cell tropism and pathogenicity of hemotrophic mycoplasma / A. Sokoli [et al.] //Vet. Res. - 2013.-Vol. 44:6 (February). - URL: http://www.veterinaryresearch.Org/content/44/l/6.

6. Prevalence of Mycoplasma suis (Eperythrozoon suis) infection in swine and swine-farm workers in Shanghai, China / Cong L. Yuan [et al.] // Am. J. Vet. Res. - 2009.- Vol. 70, -P. 890-894.

7. Prevalence of Mycoplasma suis in slaughter pigs, with correlation of PCR results to hematological findings/ M. Ritzmann [etal.]//Vet. Microbiol. - 2009.-Vol. 133.- P. 84-91.

8. Prevalence of swine hemoplasmas revealed by realtime PCR using 16S rRNA gene primers / Y. Watanabe [et a I.] // J. Vet. Med. Sei. - 2012. - Vol. 74, № 10. - P. 1315-1318.


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Для цитирования: ., . . Ветеринария сегодня. 2015;(1):47-50.

For citation: Biryuchenkova M.V., Timina A.M. Development of polimeraze chain reaction based test system for Eperythrozoon suis detection. Veterinary Science Today. 2015;(1):47-50. (In Russ.)

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ISSN 2304-196X (Print)
ISSN 2658-6959 (Online)