IMPLEMENTATION OF 454 LIFE SCIENCES TECHNOLOGY LABORATORY PRACTICES

Полный текст:


Аннотация

He article demonstrates possibility of using highly productive pyrophosphate sequencing, also known as 454 Life Sciences technology for investigation of highly variable and understudied infectious viral agents. Genome-wide nucleotide sequences of infectious bronchitis virus, infectious laryngotracheitis virus, fowl adenovirus C, and avian adenoassociated virus were determined. Recombinations in infectious bronchitis genome were identified, structural and point mutations in fowl adenovirus genome were detect ed. Infectious laryngotracheitis virus and avian adeno-associated virus genome structure was characterized.

Об авторах

N. Zinyakov
FGBI "ARRIAH", Vladimir
Россия


Ye. Ovchinnikova
FGBI "ARRIAH", Vladimir
Россия


S. Lazareva
FGBI "ARRIAH", Vladimir
Россия


A. Kozlov
FGBI "ARRIAH", Vladimir
Россия


I. Chvalas
FGBI "ARRIAH", Vladimir
Россия


Список литературы

1. Genetic analysis of fowl adenovirus isolate «Krasnodar 2009» / O. S. Osipova, N. G. Zinyakov, I. A.Chvala, V. V. Drygin//VestnikVeterinarii. -2014, -№3, -P. 33-37.

2. Zinyakov N. G. Detection of adeno-associated viruses using NGS-techniques//Molecular Diagnostics. - 2014.- Vol. 2, -P. 448-449.

3. Ovchinnikova Ye. V. Molecular and biological properties of IBV isolates detected in Russia in 2005-201Ί: thesis.. candidate of science (Biology).- Vladimir, 2012. -116 p.

4. Adair В. М., Fitzgerald S. D. Group 1 adenovirus infections // Diseases of Poultry / ed. Y. M. Saif, A. M. Fadly, J. R. Glisson [et al.].- 12th ed. - Ames, Iowa, - 2008.- P. 252-266.

5. Bossis I., Chiorini J. A. Cloning of an Avian Adeno-Associated Virus (AAAV) and Generation of Recombinant AAAV Pa rticles/J. Virol. - 2003,-Vol. 77. - P. 6799-6810.

6. Knopf C. W., Spies B., Kaerner H. C. The DNA replication origins of herpes simplex virus type 1 strain Angelotti // Nucleic Acids Research.- 1986.-Vol. 14, № 21.- P. 8655-8667.

7. Metagenomic analysis of the viromes of three North American bat species: viral diversity among different bat species that share a common habitat / E. F. Donaldson, A. N. Haskew, J. E. Gates [et al.] // J. Virol. - 2010.-Vol. 84. - P. 13004-13018.

8. Sequencing viral genomes from a single isolated plaque / J. DePew, B. Zhou, J. M. McCorrison [et al.] // J. Virol. - 2013. - Vol. 10 (181 ). - doi: 10.1186/1743-422X-10-181.


Дополнительные файлы

Для цитирования: ., ., ., ., . . Ветеринария сегодня. 2015;(1):40-44.

For citation: Zinyakov N.G., Ovchinnikova Y.V., Lazareva S.P., Kozlov A.A., Chvalas I.A. IMPLEMENTATION OF 454 LIFE SCIENCES TECHNOLOGY LABORATORY PRACTICES. Veterinary Science Today. 2015;(1):40-44. (In Russ.)

Просмотров: 65

Обратные ссылки

  • Обратные ссылки не определены.


Creative Commons License
Контент доступен под лицензией Creative Commons Attribution 4.0 License.


ISSN 2304-196X (Print)
ISSN 2658-6959 (Online)