Biological properties of foot-and-mouth disease virus А 2205/G-IV strain

According to the World Organisation for Animal Health, foot-and-mouth disease (FMD) is regularly reported in domestic and wild cloven-hoofed animals in Africa. G-I, G-IV, G-VI, G-VII, ASIA/Iran-05 genetic lineages of serotype А FMD virus are considered to be the most widespread on the African continent. Given the close economic and trade relations maintained by the Russian Federation with the countries of North Africa, of particular interest for us is studying the FMD virus of serotype A G-IV genetic lineage, which has been responsible for the infection outbreaks in the naturally susceptible animal population of the said region every year since 2012, and there is a risk of introduction of this virus genotype in to the Russian Federation. Therefore, the issues of FMD introduction risk assessment and timely diagnosis are relevant for the Veterinary Service of Russia. FMD virus A 2205/G-IV strain tested for its biological and antigenic properties in cell cultures and naturally susceptible animals (cattle and pigs) was adapted for its reproduction in initially trypsinized porcine kidney (PK) cell culture, continuous monolayer cell cultures (IB-RS-2, PSGK-30, YaDK-04, ВНК-21) by five serial passages. The virus was considered to be adapted when 90–95% cytopathic effect developed within 14–19 hours after the cell culture infection. The virus adapted to the cell cultures was tested for its infectivity with microtitration in IB-RS-2 cell culture. The virus strain tested for vaccine matching with microneutralization test (MNT) demonstrated significant difference from production A/Turkey/06, А22 No. 550/Azerbaijan/64, A22/Iraq/64, A/Iran/97, A No. 2155/Zabaikalsky/2013, A No. 2166/Krasnodarsky/2013, A No. 2269/ARRIAH/2015 strains of FMD virus.

1. Burdov A. N., Dudnikov A. I., Malyarets P. V., Ivanyushenkov V. N., RakhmanovA.M., ShazhkoZh.A. Foot-and-mouth disease. Ed. by A.N.Burdov. Moscow: Agropromizdat; 1990. 320 p. (inRuss.)

2. ZharovA. V. Pathological anatomy of animals: textbook. 2nded., revised and supplemented. Saint Petersburg: Lan’; 2013. 608 p. (in Russ.)

3. GrubmanM. J., BaxtB. Foot-and-mouth disease. Clin. Microbiol. Rev. 2004; 17 (2): 465–493. DOI: 10.1128/CMR.17.2.465-493.2004.

4. Knight-Jones T. J. D., Rushton J. The economic impacts of foot and mouth disease– what are they, how big are they and where do they occur? Prev. Vet. Med. 2013; 112(3–4): 161–173. DOI:1016/j.prevetmed.2013.07.013.

5. JamesA.D., RushtonJ. The economics of foot and mouth disease. Rev. Sci. Tech. 2002; 21(3): 637–644. DOI: 10.20506/rst.21.3.1356.

6. WOAH. Terrestrial Animal Health Code. Режим доступа: https://www.woah.org/en/what-we-do/standards/codes-and-manuals/terrestrial-code-online-access.

7. Brooksby J. B. The virus of foot-and-mouth disease. Adv. Virus Res. 1958; 5: 1–37. DOI: 10.1016/s0065-3527(08)60670-3.

8. CarrilloC., TulmanE. R., DelhonG., LuZ., CarrenoA., VagnozziA., etal. Comparative genomics of foot-and-mouth disease virus. J. Virol. 2005; 79(10): 6487–6504. DOI: 10.1128/JVI.79.10.6487-6504.2005.

9. DuchatelF., BronsvoortB.M.deC., LycettS. Phylogeographic analysis and identification of factors impacting the diffusion of foot-and-mouth disease virus in Africa. Front. Ecol. Evol. 2019; 7:371. DOI:10.3389/fevo.2019.00371.

10. JamalS.M., BelshamG.J. Foot-and-mouth disease: past, present and future. Vet. Res. 2013; 44(1):116. DOI: 10.1186/1297-9716-44-116.

11. Jori F., Caron A., Thompson P. N., Dwarka R., Foggin C., de Garine-WichatitskyM.,etal. Characteristics of foot-and-mouth disease viral strains circulating at the wildlife/livestock interface of the Great Limpopo Transfrontier Conservation Area. Transbound. Emerg. Dis. 2016; 63 (1): e58– e70. DOI: 10.1111/tbed.12231.

12. TekleghiorghisT., MoormannR.J.M., WeerdmeesterK., DekkerA. Foot-and-mouth disease transmission in Africa: Implications for control, a review. Transbound. Emerg. Dis. 2016; 63(2): 136–151. DOI:10.1111/tbed.12248.

13. Maree F. F., Kasanga C. J., Scott K. A., Opperman P. A., Chitray M., SangulaA. K., et al. Challenges and prospects for the control of foot-and-mouth disease: an African perspective. Vet. Med. (Auckl.). 2014; 5: 119–138. DOI: 10.2147/VMRR.S62607.

14. Bouguedour R., Ripani A. Review of the foot and mouth disease situation in North Africa and the risk of introducing the disease into Europe. Rev. Sci. Tech. Off. Int. Epiz. 2016; 35(3): 757–768. DOI:10.20506/rst.35.3.2566.

15. McLachlan I., Marion G., McKendrick I. J., Porphyre T., Handel I. G., BronsvoortB.M. Endemic foot and mouth disease: pastoral in-herd disease dynamics in sub-Saharan Africa. Sci. Rep. 2019; 9 (1):17349. DOI: 10.1038/s41589-019-53658-5.

16. ValarcherJ. F., LeforbanY., RweyemamuM., RoederP. L., GerbierG., MackayD. K., etal. Incursions of foot-and-mouth disease virus into Europe between 1985 and 2006. Transbound. Emerg. Dis. 2008; 55 (1): 14–34. DOI: 10.1111/j.1865-1682.2007.01010.x.

17. Pezzoni G., Bregoli A., Grazioli S., Barbieri I., Madani H., OmaniA., etal. Foot-and-mouth disease outbreaks due to an exotic virus serotypeA lineage (A/AFRICA/G-IV) in Algeria in2017. Transbound. Emerg. Dis. 2019; 66 (1): 7–13. DOI: 10.1111/tbed.13017.

18. Ehizibolo D. O., De Vleeschauwer A. R., Haegeman A., Lefebvre D., Nwosuh C. I., Umoh J. U., et al. Serological and molecular epidemiology of foot-and-mouth disease viruses in agro-pastoralist livestock herds in the kachia grazing reserve, Nigeria. Transbound. Emerg. Dis. 2019; 66 (4): 1575–1586. DOI: 10.1111/tbed.13182.

19. Gizaw D., Tesfaye Y., Wood B. A., Di Nardo A., Shegu D., Muluneh A., et al. Molecular characterisation of foot-and-mouth disease viruses circulating in Ethiopia between 2008 and 2019. Transbound. Emerg. Dis. 2020; 67 (6): 2983–2992. DOI: 10.1111/tbed.13675.

20. Lycett S., Tanya V. N., Hall M., King D. P., Mazeri S., Mioulet V., et al. Theevolution and phylodynamics of serotypeA and SAT2 foot-and-mouth disease viruses in endemic regions of Africa. Sci. Rep. 2019; 9 (1):5614. DOI: 10.1038/S41598-019-41995-4.

21. RweyemamuM. M. Antigenic variation in foot-and-mouth disease: studies based onthevirus neutralization reaction. J.Biol. Stand. 1984; 12(3): 323–337. DOI: 10.1016/s0092-1157(84)80013-x.

22. WRLFMD Quarterly Report. Foot-and-mouth disease: April to June 2019. Режим доступа: https://www.wrlfmd.org/sites/world/files/quick_media/OIE-FAO%20FMD%20Ref%20Lab%20Report%20Apr%20-%20Jun%202019.pdf.