Properties of Actinobacillus pleuropneumoniae isolates

Results of tests of six Actinobacillus pleuropneumoniae isolates recovered from the diseased pigs kept in animal holdings located in the Russian Federation for their biological properties (biochemical, proteomic, antigenic and pathogenic ones) are presented in the paper. Proteomic properties were determined with mass-spectrometry using Autof MS 1000 mass-spectrometer (Autobio Diagnostics Co., Ltd, China): protein profiles were plotted and the peaks characteristic for each isolate were identified. Mass-spectra of tested Actinobacillus isolates and reference Actinobacillus pleuropneumoniae DSM 13472 strain were found to be in the m/z range of 2,000–12,000 Da. The following peaks (m/z) were common for all Actinobacillus pleuropneumoniae isolates and the strain: 2,541 ± 2; 4,267 ± 2; 5,085 ± 2; 6,450 ± 2; 7,207 ± 4; 9,408 ± 3; 11,820 ± 6. Therewith, the highest intensity (100%) was reported for the peak at 5,085 ± 2, that was supposed to be a specific feature of Actinobacillus pleuropneumoniae. All isolates were confirmed to belong to Actinobacillus pleuropneumoniae species and to 2, 5 and 9 serotypes by real-time polymerase chain reaction using species-specific and serotype-specific primers. Actinobacillus рleuropneumoniae isolates were tested for their pathogenic properties by experimental infection of white mice and 2.5–3 month-old piglets. All tested isolates were pathogenic for both white mice and piglets. Isolate No. 4 belonging to serotype 5 was found to be the most virulent out of tested isolates. Thus, LD₅₀ was 4.19 lg microbial cells for white mice and 5.49 lg microbial cells for piglets that was consistent to the data of other authors carried out tests of actinobaccilli isolated in the Russian Federation for their pathogenicity. The isolates were deposited to the FGBI “ARRIAH” Collection of Microorganism Strains.

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