Bovine leukosis incidence in Republic of Dagestan in 2021

Results of epizootological analysis of data on bovine leucosis (BL) incidence in the Republic of Dagestan in 2021 are reported. Bovine blood was diagnostically tested for leukosis in 32 veterinary laboratories and diagnostic offices including SBI RD Republican Veterinary Laboratory. 720,489 sera were serologically tested and 7,188 (1.0%) samples were serologically positive for bovine leukaemia virus (BLV). Among the infected animals, 527 ones were subjected to haematologic testing. Persistent leukocytosis was reported in 153 (29.03%) blood samples of haematologically tested BL diseased cattle. Statistical analysis of BLV prevalence in the republic was performed for 41 Raions and 8 municipalities. High percentage of BLV infection in the animal population was reported in fourteen Raions: Kumtorkalinsky (5.8%), Gunibsky (5.3%), Tarumovsky (3.3%), Karabudakhentsky (2.9%), Akhvakhsky (2.0%), Kizlyarsky (1.8%), Charodinsky (1.7%), Kazbekovsky (1.6%), Babayurtovsky (1.5%), Tlyaratinsky (1.1%), Dakhadayevsky (1.04%), Sergokalinsky (1.02%), Novolaksky (1.0%), Shamilsky (1.0%), and in four municipalities: Makhachkala (2.0%), Izberbash (1.14%), Khasavyurt (1,1%) and Yuzhno-Sukhokumsk (1.0%). In 21 Raions and two municipalities, BLV seropositivity was below 1.0%. No BLV infected animals were detected in the Agulsky, Akhtynsky, Dokuzparinsky, Magaramkentsky, Khivsky, Suleiman-Stalsky Raions and in Derbent and Dagestanskiye Ogni municipalities. Studies of BLV prevalence in 2015–2021 demonstrated that the highest level of the animal infection was reported in 2015 (13.9%) and the lowest – in 2021 (1.0%). However, the number of animals serologically tested in 2021 exceeded the number of animals tested over the whole study period. Therefore, the Republic of Dagestan remains BL infected region.

1. Olaya-Galán N. N., Corredor-Figueroa A. P., Guzmán-Garzón T. C., Ríos-Hernandez K. S., Salas-Cárdenas S. P., Patarroyo M. A., Gutierrez M. F. Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption. Epidemiol. Infect. 2017; 145 (15): 3125–3130. DOI:10.1017/S0950268817002229.

2. Zhang R., Jiang J., Sun W., Zhang J., Huang K., Gu X., et al. Lack of association between bovine leukemia virus and breast cancer in Chinese patients. Breast Cancer Res. 2016; 18 (1):101. DOI:10.1186/s13058-016-0763-8.

3. Mustafayev A. R. Immunodiffusion assay as a method of bovine leukosis post-mortem diagnosis. Veterinary Science Today. 2022; 11 (1): 49–52. DOI:10.29326/2304-196X-2022-11-1-49-52.

4. Simonyan G. A., Khisamudinov F. F. Veterinary hematology. Moscow: Kolos; 1995. 256 p. (in Russ.)

5. Mustafaev A. R., Ivashov E. A. Dynamics of incidence of cattle with leukosis virus on the background of gamma radiation in the territory of the Republic of Dagestan after the Chernobyl accident. Veterinarian. 2021; 5: 30–38. DOI:10.33632/1998-698X.2021-5-30-38. (in Russ.)

6. Mirsky M. L., Olmstead C. A., Da Y., Lewin H. A. The prevalence of proviral bovine leukemia virus in peripheral blood mononuclear cells at two subclinical stages of infection. J. Virol. 1996; 70 (4): 2178–2183. DOI:10.1128/JVI.70.4.2178-2183.1996.

7. Nekoei S., Hafshejani T. T., Doosti A., Khamesipour F. Molecular detection of bovine leukemia virus in peripheral blood of Iranian cattle, camel and sheep. Pol. J. Vet. Sci. 2015; 8 (4): 703–707. DOI:10.1515/pjvs-2015-0091.

8. Donnik I. M., Ponomareva O. I., Krivonos R. A., Lysenko A. A., Koshchaev A. G., Chernykh O. Yu., et al. Elimination of bovine leukemia in industrial production conditions. Veterinaria Kubani. 2021; 2: 3–8. DOI:10.33861/2071-8020-2021-2-3-8. (in Russ.)

9. Gulykin M., Barabanov I., Ivanova L., Stepanova T., Kozireva N., Simonian G., et al. Monitoring of epidemiologic situation with Bovine Leukemia in production and breeding herds of Russian Federation in 2014–2015. Veterinaria i kormlenie. 2016; 4: 5–41. Available at: http://vetkorm.ru/magasines/veterinariya-i-kormlenie-4-ijul-avgust-2016g. (in Russ.)

10. Mischenko V. A., Petrova O. N., Karaulov A. K., Mischenko A. V. The problem of bovine leukemia. Vladimir: FGBI “ARRIAH”; 2018. 38 p. Available at: https://www.arriah.ru/upload/iblock/ff2/ff282f8bce04ee91ae62653b6f608805.pdf. (in Russ.)

11. Gillet N., Florins A., Boxus M., Burteau C., Nigro A., Vandermeers F., et al. Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human. Retrovirology. 2007; 4:18. DOI:10.1186/1742-4690-4-18.

12. Wu D., Takahashi K., Murakami K., Tani K., Koguchi A., Asahina M., et al. B-1a, B-1b and conventional B cell lymphoma from enzootic bovine leukosis. Vet. Immunol. Immunopathol. 1996; 55 (1–3): 63–72. DOI:10.1016/s0165-2427(96)05631-0.

13. Donnik I. M., Gulyukin M. I., Busol V. A., Kovalenko L. V., Kovalenko A. M. Bovine leukemia virus infection – diagnostics, eradication, and anthropozoonotic potential (background) (review). Agricultural biology. 2021; 56 (2): 230–244. DOI:10.15389/agrobiology.2021.2.230eng.

14. Makarov V. V., Lozovoy D. A. Epizootological features of modern cattle leukemia. Vestnik of the Russian agricultural science. 2020; 1: 53–58. DOI:10.30850/vrsn/2020/1/53-58. (in Russ.)

15. Methodical instructions for diagnosis of bovine leucosis: approved by Veterinary Department of the RF Ministry of Agriculture on August 23, 2000 No. 13-7-2/2130. Available at: http://docs.cntd.ru/document/1200118749. (in Russ.)

16. Methodical instructions for epidemiological study of bovine leucosis: approved by academician A. M. Smirnov, Veterinary Medicine Department of the Russian Academy of Agricultural Sciences, on June 19, 2001. Мoscow; 2001. 28 p. (in Russ.)

17. Mustafaev A., Gulyukin M., Salikhov Yu. Monitoring of the spread of bovine leukemia virus in the Republic of Dagestan in 2018. Veterinaria i kormlenie. 2019; 4: 18–20. DOI:10.30917/ATT-VK-1814-9588-2019-4-5. (in Russ.)

18. Mustafayev A. R., Ivashev E. A. Influence of radioactive isotopes 90Sr, 137Cs, 210Pb on environmental objects and their relationship with bovine leukemia in ecosystems of the Republic of Dagestan. South of Russia: ecology, development. 2021; 16 (4): 136–145. DOI:10.18470/1992-1098-20214-136-145. (in Russ.)

19. Mustafayev A. R., Gulyukin M. I., Gaydarbekova H. M. The analysis of the epizootic situation of the virus of cattle leukosis in the Republic of Dagestan. Veterinaria i kormlenie. 2017; 5: 25–27. Available at: http://vetkorm.ru/magasines/veterinariya-i-kormlenie-5-sentyabr-oktyabr-2017g. (in Russ.)

20. Mustafayev A. R. Epidemic situation on enzootic bovine leukosis in public and individual farms in the Republic of Dagestan. Veterinary Science Today. 2021; (2): 144–150. DOI:10.29326/2304-196X-2021-2-37-144-150.