ASSESSMENT OF EFFICACY AND SAFETY OF INACTIVATED RABIES VACCINES BASED ON “ARRIAH” STRAIN AND FORMULATED WITH DIFFERENT ADJUVANTS IN CATTLE

Rabies cases are still reported in various mammal species in the Russian Federation. Wild carnivores contacting with domestic and farm animals and transmitting the pathogen to them are the main source and natural reservoirs of rabies. The highest level of rabies incidence in livestock is observed among cattle. Thus, 158 new outbreaks were reported in the Russian Federation in 2017 where the virus was identifi ed in cattle. Rabies specifi c prophylaxis as well as assessment of its effi cacy based on the level of accumulated antirabies virus neutralizing antibodies currently occupy one of the leading positions in the modern system of veterinary and sanitary measures against rabies. The paper presents the study results confi rming effi cacy and safety of three inactivated rabies vaccines based on the “ARRIAH” strain and intended for rabies prevention in cattle. The adsorbed and two emulsion rabies vaccines, formulated with diff erent adjuvants demonstrated innocuity and safety, and induced post-vaccination immunity in all immunized animals at day 21 post inoculation. The mean titers of anti-rabies antibodies were as follows: for inactivated adsorbed vaccine – 4.02 ± 0.76 IU/cm³; for inactivated emulsion vaccines – 16.30 ± 2.03 and 20.73 ± 3.39 IU/cm³. As compared with the adsorbed vaccine, the emulsion vaccines formulated with Montanide ISA 206 and ISA 70 adjuvants induce stronger immunity.

1. Adjuvants: monograph [Ad”yuvanty: monografi ya]. А. Ya. Samuylenko, S. А. Grin, V. I. Yeremetz [et al.]; ed. by А. Ya. Samuylenko. М.: VNITIBP, 2016 (in Russian).

2. Immunogenicity analysis of the vaccine used for rabies prevention in the territory of the Russian Federation [Analiz immunogennosti vakcin, primenyaemyh dlya profi laktiki beshenstva zhivotnyh na territorii Rossijskoj Federacii]. Ye. V. Chernyshova, N. А. Nazarov, А. Ye. Metlin [et al.]. Proceedings of the Federal Centre for Animal Health. 2010; 8: 64–73 (in Russian).

3. Analysis of the effi cacy of oral rabies vaccination in wild nature based on the example of some regions in the Russian Federation [Analiz eff ektivnosti oral’noj vakcinacii protiv beshenstva v dikoj srede na primere nekotoryh regionov Rossijskoj Federacii]. А. Yu. Sukharkov, N. А. Nazarov, А. Ye. Metlin [et al.]. Proceedings of the Federal Centre for Animal Health. 2010; 8: 57–63 (in Russian).

4. Borisov А. V. Development of production technology for rabies vaccines in wild carnivores [Razrabotka tekhnologii izgotovleniya virusvakcin protiv beshenstva dikih plotoyadnyh]: author’s abstract. … candidate of veterinary science. Vladimir, 2003 (in Russian).

5. Glanz S. Medical and biological statistics [Mediko-biologicheskaya statistika]: translated from English. М.: Praktika, 1999 (in Russian).

6. Gruzdev К. N., Metlin А. Ye. Animal rabies [Beshenstvo zhivotnyh]. Vladimir: FGBI “ARRIAH”, 2019 (in Russian).

7. Study of colostral immunity in the off spring of raccoon dogs immunized with the “Sinrab” oral rabies virus vaccine [Izuchenie kolostral’nogo immuniteta u potomstva enotovidnyh sobak, immunizirovannyh oral’noj antirabicheskoj virusvakcinoj «Sinrab»]. А. V. Borisov, I. А. Domskiy, V. V. Mikhalishin, А. N. Balashov. Veterinaria i kormlenie. 2013; 5: 6–8 (in Russian).

8. Mikhalishin V. V., Mamkov N. S. Adjuvants and their use [Ad”yuvanty i ih ispol’zovanie]. Proceedings of the Federal Center for Animal Health. 2008; 6: 340–371 (in Russian).

9. Nedosekov V. V. Modern vaccines against animal rabies [Sovremennye vakciny protiv beshenstva zhivotnyh]. Veterinariya. 2003; 8: 23–25 (in Russian).

10. Oral vaccination of wild cornivores against rabies [Oral’naya vakcinaciya dikih plotoyadnyh zhivotnyh protiv beshenstva]. А. Ye. Metlin, S. S. Rybakov, V. V. Mikhalishin, T. N. Muller. Veterinariya. 2009; 8: 18–25 (in Russian).

11. Evaluation of palatability of oral rabies vaccines [Ocenka poedaemosti oral’nyh antirabicheskih vakcin]. А. Yu. Sukharkov, Е. Yu. Chernyshova, А. Ye. Metlin [et al.]. Veterinariya. 2011; 11: 31–34 (in Russian).

12. Chernyshova Ye. V., Nazarov N. А., Metlin А. Ye. Methodical guidelines for determination of virus-neutralizing antibodies against rabies virus in animal sera using neutralization test in BHK-21 cell culture (FAVN) [Metodicheskie ukazaniya po opredeleniyu virusnejtralizuyushchih antitel k virusu beshenstva v syvorotkah krovi zhivotnyh v reakcii nejtralizacii v kul’ture kletok VNK-21 (FAVN)]. Methodical guidelines for diagnosis of livestock diseases using serological tests [Metodicheskie ukazaniya po diagnostike zabolevanij sel’skohozyajstvennyh zhivotnyh s ispol’zovaniem serologicheskih reakcij]. FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”). Vladimir, 2015; 207–216 (in Russian).

13. Rabies epidemic situation in the Russian Federation in October 2018 [Epizooticheskaya obstanovka po beshenstvu na territorii Rossii v oktyabre 2018 goda]. Rosselkhoznadzor. URL: https://www.fsvps.ru/fsvps/news/28513.html (date of reference: 30.10.18) (in Russian).

14. Rabies epidemic situation in the Russian Federation in 2017 [Epizooticheskaya situaciya v Rossijskoj Federacii, 2017 god]. compiler: О. N. Petrova, А. К. Karaulov, N. S. Bardina [et al.]. Vladimir: FGBI “ARRIAH”, 2018. URL: http://www.fsvps.ru/fsvps-docs/ru/iac/rf/2017/iac2017.pdf (date of access: 01.11.18) (in Russian).

15. Aubert M. F. A. Methods for the calculation of titres. Laboratory Techniques in Rabies. ed. F. X. Meslin, M. Kaplan, H. Koprowski. 4th ed. Geneva: WHO, 1996; 445–459.

16. International Committee on Taxonomy of Viruses (ICTV). URL: https://talk.ictvonline.org/taxonomy/.

17. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (Mammals, Birds and Bees). OIE. 8th ed. Paris, 2018; 1, Chap. 3.1.17: 578– 612. URL: https://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/3.01.17_RABIES.pdf.