METHOD FOR FMD VIRUS 146S COMPONENT CONCENTRATION DETERMINATION WITH REAL-TIME REVERSE TRANSCRIPTION – POLYMERASE CHAIN REACTION IN VACCINE RAW MATERIALS


https://doi.org/10.29326/2304-196X-2018-2-25-31-35

Полный текст:


Аннотация

Method for determination of foot-and-mouth disease (FMD) virus 146S component concentration with real-time reverse transcription – polymerase chain reaction (rtRT-PCR) in vaccine raw materials is developed. Negative regression model of  С146S = (30.269 – Ct)/4.155 type allowing determination of FMDV 146S particle concentrations based on the amplification threshold cycle values (Ct) is proposed. It has been experimentally proven that the quantity of the 146S component determined by the real-time RT-PCR method using developed regression model and contained in the inoculation dose of FMD vaccine confers protection to the vaccinated animals against generalized FMD of A, O, Asia-1 types. rtRT-PCR method is highly sensitive and allows rapid and highly reliable estimation of  the 146S antigen concentration in FMD vaccine. The method for 146S particle quantity determination by real-time RT-PCR using the regression model is reliable and demonstrates high correlation (95.5–99.0%) with the complement fixation test results.


Об авторах

M. I. Doronin
FGBI ARRIAH
Россия

Researcher, Candidate of Science (Biology).

Vladimir



А. М. Timina
FGBI ARRIAH
Россия

Leading Researcher, Candidate of Science (Veterinary Mediciane).

Vladimir


D. А. Lozovoy
FGBI ARRIAH
Россия

Director, Candidate of Science (Veterinary Medicine).

Vladimir


V. А. Starikov
FGBI ARRIAH
Россия

Leading Researcher, Candidate of Science (Veterinary Medicine).

Vladimir


D. V. Mikhalishin
FGBI ARRIAH
Россия

Head of  Laboratory, Candidate of Science (Veterinary Medicine).

Vladimir


N. N. Medvedeva
FGBI ARRIAH
Россия

Biologist.

Vladimir


А. V. Borisov
FGBI ARRIAH
Россия

Leading Researcher, Candidate of Science (Veterinary Medicine).

Vladimir


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Дополнительные файлы

Для цитирования: Doronin M.I., Timina А.М., Lozovoy D.А., Starikov V.А., Mikhalishin D.V., Medvedeva N.N., Borisov А.V. METHOD FOR FMD VIRUS 146S COMPONENT CONCENTRATION DETERMINATION WITH REAL-TIME REVERSE TRANSCRIPTION – POLYMERASE CHAIN REACTION IN VACCINE RAW MATERIALS. Ветеринария сегодня. 2018;(2):31-35. https://doi.org/10.29326/2304-196X-2018-2-25-31-35

For citation: Doronin M.I., Timina A.M., Lozovoy D.A., Starikov V.A., Mikhalishin D.V., Medvedeva N.N., Borisov А.V. METHOD FOR FMD VIRUS 146S COMPONENT CONCENTRATION DETERMINATION WITH REAL-TIME REVERSE TRANSCRIPTION – POLYMERASE CHAIN REACTION IN VACCINE RAW MATERIALS. Veterinary Science Today. 2018;(2):31-35. https://doi.org/10.29326/2304-196X-2018-2-25-31-35

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