TESTING OF EMULSION VACCINES BASED ON WHITE OILS OBTAINED AS A RESULT OF HYDROCATALYTIC PROCESSING OF OILSTOCK

The paper demonstrates the possibility to use white mineral oils resulting from hydrocatalytic processing of unconverted vacuum gas oil as a basis for an oil adjuvant used in the production of FMD vaccines intended for livestock. Samples of the developed white oils were tested for their reactogenicity and pyrogenicity, and emulsion vaccines were tested for their reactogenicity, potency, and emulsion stability. Tests results were compared with the ones for a foreign analogue. Blood samples were taken from animals before vaccination and 14 days after it to obtain sera which were further tested for FMD antibodies using VNT. Assessment of the white oil sample reactogenicity demonstrated that sample BM 1.4 obtained during hydroisomerization was the least reactogenic. Sample V-BM 1.4 demonstrated the best result in comparison with other samples within the group as well as in comparison with the control sample during the emulsion stability test. According to the potency test results, the emulsion vaccine based of the white oil sample BM 1.4 demonstrated the greatest difference in antibody titers in 14 days - 4.15 log₂, while the one based on the control sample - 3.50 log₂. This means that it the use of this vaccine results in 17.7-fold increase in FMD antibodies, while the use of control emulsion vaccine in 11.3-fold increase. Vaccines based on BM 1.3 and BM 2.2 oil samples demonstrated lower performance in comparison with the control vaccine in terms of these parameters.

белые минеральные масла, масляные адъюванты, эмульсионные вакцины, гидрокаталитические процессы, white mineral oils, oil adjuvants, emulsion vaccines, hydrocatalytic processes

1. Адъюванты в современной вакцинологии / Е. Ю. Исаенко, Е. М. Бабич, И. В. Елисеева [и др.] // Анналы Мечниковского института. - 2013. - № 4. - С. 5-21.

2. Белые масла - производство, требования, технологии / С. В. Заглядова [и др.] // II Ежегодная междунар. конф. «Масла и топлива СНГ», 27-29 мая 2014 г.

3. Большой медицинский энциклопедический словарь / под ред. В. И. Бородулина. - 4-е изд., испр. и доп. - М.: Рипол Классик, 2007. - 960 с.

4. Мамков Н. С. Масляные адъюванты для противоящурных вакцин: дис.. д-ра вет. наук. - Владимир, 1999. - 255 с.

5. Медуницын Н. В. Вакцинология. - 2-е изд., перераб. и доп. - М.: Триада-Х, 2004. - 448 с.

6. Михалишин В. В., Мамков Н. С. Адъюванты и их использование // Тр. Федерального центра охраны здоровья животных: матер. Междунар. науч. конф. «Инфекц. патология ж-ных», посвящ. 50-летию ФГУ «ВНИИЗЖ». - 2008. - Т. 6. -С. 340-371.

7. Способ получения маловязких белых масел: пат. 2549898 Российская Федерация / Е. В. Кашин, С. В. Заглядова, Т. Н. Шабалина [и др.]; ОАО «Нефтяная компания «Роснефть». - № 2014105866/04; заявл. 18.02.14; опубл. 10.05.2015.

8. ФС.2.2.0004.15 Масло вазелиновое // Государственная фармакопея Российской Федерации. - Т. 3. - 13-е изд. -М.: ФЭМБ, 2015. - 1292 с.

9. Шабалина Т. Н. Разработка технологии производства нефтяных маловязких масел с применением гидрокаталитических процессов: дис.. д-ра техн. наук. - Москва, 1999. -361 с.

10. European Pharmacopeia 8.0. Vol. 1-2 / Directorate for the Quality of Medicines & Healthcare of the Council of Europe (EDQM). - 8th ed. - Strasbourg, 2014.

11. FDA 21 CFR 172.878. Food additives permitted for direct addition to food for human consumption. White mineral oil. -URL: https://www.accessdata.fda.gov/SCRIPTs/cdrh/cfdocs/cfcfr/CFRSearch.cfm?fr=172.878.

12. FDA 21 CFR 178.3620. Indirect food additives: adjuvants, production aids and sanitizers. Mineral oil. - URL: https://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/CFRSearch.cfm?FR=178.3620.

13. The United States Pharmacopeia 38 - National Formulary 33. - 38th еd. - 2015.

14. Vaccine Adjuvants: Preparation Methods and Research Protocols / ed. D. T. O'Hagan // Methods in Molecular Medicine / ed. J. M. Walker. - Totowa, NJ: Humana Press, Inc., 2000. - Vol. 42. -342 р.