ASSESSMENT OF POSSIBLE USE OF CARBON ISOTOPE RATIOS IN AMINO ACIDS FOR MEAT PRODUCT GEOGRAPHICAL ORIGIN IDENTIFICATION

Полный текст:


Аннотация

Possible use of compound-specific isotope analysis (GC-C-IRMS) was assessed using 255 meat samples from 24 countries. Upon results of isotope ratio (δ13C) determination in amino acids after gas chromatographic separation samples were classified using Support Vector Machines and linear discriminate analysis. Prognosis accuracy was 65-75%. Despite the fact that the use of compound analysis only provides low identification accuracy there's a possibility to considerably increase reliability of classification in combination with other data by means of extending dimension by the number of variables.

Об авторах

A. Solovyev
FGBI “ARRIAH”
Россия


I. Podkolzin
FGBI “ARRIAH”
Россия


Список литературы

1. Amrani A., Sessions A. L., Adkins J. F. Compound-Specific 534S Analysis of Volatile Organics by Coupled GC/Multicollector-ICPMS // Anal. Chem. - 2009. - Vol. 81, № 21. - P. 9027-9034; doi:10.1021/ac9016538.

2. Characterisation of milk by analysis of its terpene fractions / C. Fernandez, C. Astier, E. Rock [et al.] // Int. J. Food Sci. Tech. - 2003. - Vol. 38. - P. 445-451.

3. High-precision continuous-flow isotope ratio mass spectrometry / J. T. Brenna, T. N. Corso, H. J. Tobias, R. J. Caimi // Mass Spectrom. Rev. - 1988. - Vol. 16, № 5. -P. 227-258.

4. Hofmann D., Gehre M., Jung K. Sample preparation techniques for the determination of natural 15 N/14 N variations in amino acids by gas chromatography-com-bustion-isotope ratio mass spectrometry (GC-C-IRMS) // Isot. Environ. Healt. S. - 2003. - Vol. 39, № 3. - P. 233-244.

5. Kelly S., Heaton K., Hoogewerff J. Tracing the geographical origin of food: The application of multi-element and multi-isotope analysis // Trends Food Sci. Tech. -2005. - Vol. 16, № 12. - P. 555-567.

6. Package for Kernel Methods in {R} / A. Karatzoglou, A. Smola, K. Hornik, A. Zeileis // J. Stat. Softw. - 2004. -Vol. 11, № 9; doi:10.18637/jss.v011.i09.

7. Luykx D. M. A. M., van Ruth S. M. An overview of analytical methods for determining the geographical origin of food products // Food Chem. - 2008. - Vol. 107, № 2. -P. 897-911.

8. Meier-Augenstein W. Stable isotope analysis of fatty acids by gas chromatography-isotope ratio mass spectrometry // Anal. Chim. Acta. - 2002. - Vol. 465, № 1-2. -P. 63-79.

9. Modern Applied Statistics with S / ed. W. N. Venables, B. D. Ripley. - 4th ed. - New York: Springer, 2002. - URL: http://www.stats.ox.ac.uk/pub/MASS4.

10. Package 'caret': Classification and Regression Training / M. Kuhn [et al.]. - 2016. - URL: http://cran.r-project. org/package=caret.

11. Package 'e1071': Misc Functions of the Department of Statistics, Probability Theory Group (Formerly: E1071), TU Wien / D. Meyer [et al.]. - 2017. - URL: https://CRAN.R-project.org/package=e1071.

12. pROC: an open-source package for R and S+ to analyze and compare ROC curves / X. Robin [et al.] // BMC Bioinformatics. - 2011. - Vol. 12:77; doi:10.1186/1471-2105-12-77.

13. Relationships between flavoring capabilities, bacterial composition, and geographical origin of natural whey cultures used for traditional waterbuffalo mozzarella cheese manufacture / G. Mauriello, L. Moio, A. Genovese, D. Ercolini // J. Dairy Sci. - 2003. - Vol. 86. -P. 486-497.

14. Sarkar D. Lattice: Multivariate Data Visualization with R. - New York: Springer, 2008. - 273 p. - URL: http://lmdvr.r-forge.r-project.org.

15. Wickham H. ggplot2: Elegant Graphics for Data Analysis (Use R!). - New York: Springer, 2009. - URL: http://doi.org/10.1007/978-0-387-98141-3.


Дополнительные файлы

Для цитирования: ., . . Ветеринария сегодня. 2017;(2):10-12.

For citation: Solovyev A.I., Podkolzin I.V. ASSESSMENT OF POSSIBLE USE OF CARBON ISOTOPE RATIOS IN AMINO ACIDS FOR MEAT PRODUCT GEOGRAPHICAL ORIGIN IDENTIFICATION. Veterinary Science Today. 2017;(2):10-12. (In Russ.)

Просмотров: 61

Обратные ссылки

  • Обратные ссылки не определены.


Creative Commons License
Контент доступен под лицензией Creative Commons Attribution 4.0 License.


ISSN 2304-196X (Print)
ISSN 2658-6959 (Online)