Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine sera
Abstract
Indirect ELISA-based test-system was developed for determination of Mannheimia haemolytica antibody titre in sera from cattle of different age-sex groups. Lipopolysaccha-ride antigen (LPS-antigen) produced by phenol-water extraction from Mannheimia haemolytica strain No. 29696 (ATCC collection) was used as an antigen. Results obtained during the comparative examination of bovine sera using the proposed test-system and commercial test-system «Mannheimia haemolytica ELISA KIT» (BIOX, Belgium) are demonstrated. Relative specificity and sensitivity of the designed method were determined.
About the Authors
Ye. S. Kostrova
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
O. P. Bjadovskaya
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
Ye. V. Peshkova
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
O. V. Pruntova
FGBI "ARRIAH", Vladimir
Russian Federation
Russian Federation
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Review
For citations:
Kostrova Ye.S., Bjadovskaya O.P., Peshkova Ye.V., Pruntova O.V. Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine sera. Veterinary Science Today. 2017;(1):34-37. (In Russ.)
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