Analysis of whole genome sequence of strain "0" of avian infectious laryngotracheitis virus

Data on identification and analysis of whole genome nucleotide sequence of strain «O» of avian infectious laryngotracheitis virus are demonstrated. In the process of the whole genome sequence mapping a number of unique and significant nucleotide properties has been identified in genes that code surface proteins responsible for interacting with cell receptors. Strain «O» has been demonstrated to belong to phylogenetic group 1 subgroup 1 (1.1). Genetic relatedness of domestic strain «0» to other vaccine strains widely used in global poultry industry such as Serva, Cover, Hudson and Fowl laryngo has been determined. The mapped nucleotide sequence has been deposited in the international GenBank database under the accession number KU128407. It is the first complete genetic characterization of the domestic vaccine strain.

вирус инфекционного ларинготрахеита, полногеномное секвенирование, последовательность полного генома, картирование генома, филогенетический анализ, infectious laryngortacheitis virus, whole genome sequencing, whole genome sequence, gene mapping, phylogenetic analysis

1. Очистка и концентрирование вируса инфекционного ларинготрахеита птиц / A.A. Козлов, Д.Б. Андрейчук, А.Н. Андриясова, Н.С. Мудрак // Инновационное развитие науки в обеспечении биологической безопасности: сб. материалов 2-й Междунар. науч. конф. молодых ученых. - Гвардейский, Казахстан, 2014. - С. 125-128.

2. Пат. 2207372 Российская Федерация, МПК7 C12N 7/00. Штамм «О» вируса инфекционного ларинготрахеита птиц для изготовления диагностических и вакцинных препаратов / A.B. Бочарников, В.Ю. Кулаков, A.B. Борисов [и др.]; ФГУ «ВНИИЗЖ». - № 2002105552/13; заявл. 28.02.2002; опубл. 27.06.2003.

3. Attenuated vaccines can recombine to form virulent field viruses / S.W. Lee, P.F. Markham, M.J. Coppo [et al.] // Science. - 2012. - Vol. 337, № 6091. - P. 188.

4. Complete genome sequence of the first Chinese virulent infectious laryngotracheitis virus / C. Kong, Y. Zhao, X. Cui [et al.] // PLoS ONE. - 2013. - Vol. 8, № 7. - URL: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0070154.

5. Genomic sequence analysis of the United States infectious laryngotracheitis vaccine strains chicken embryo origin (CEO) and tissue culture origin (TCO) / M. Garcia, V. Volkening, S.M. Riblet [et al.] // Virology. - 2013. - Vol. 440. - P. 64-74.

6. Molecular epidemiology of infectious laryngotracheitis: a review / K.R. Menendez, M. Garcia, S. Spatz, N.L. Tablante // Avian Pathology. - 2014. - Vol. 43, № 2. - P. 108-117.

7. Phylogenetic and molecular epidemiological studies reveal evidence of multiple past recombination events between infectious laryngotracheitis viruses / S.W. Lee, J.M. Devlin, J.F. Markham [et al.] // PLoS ONE. - 2013, - Vol. 8, № 2. - URL: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0055121.