The biocidal effect of a new disinfectant against microbial isolates

Introduction. Production of animal products is an essential component of the agro-industry. The concentration of numerous production facilities on animal farms, the increase in their area, high stocking density, and rapid restocking of livestock are all favorable factors for the spread of infections, leading to significant economic losses. To achieve high production targets, it is necessary to constantly maintain proper sanitary and hygienic status of animal farms, which is achieved through combined cleaning of all indoor surfaces (ceilings, walls, floors), equipment and tools, followed by mandatory disinfection. Therefore, the development and implementation of novel complex disinfectants become a relevant task, as this will ensure a high level of biological safety at agro-industrial establishments.

Objective. To study the biocidal effect of a new disinfectant against microflora from different resistance groups to chemical disinfectants under conditions simulating the production environment of livestock facilities.

Materials and methods. The biocidal effect of the new medicinal product was studied on test objects artificially contaminated with microorganisms. The test objects were made of cambric (with and without a protective substrate) as well as construction materials measuring 100 cm2 used in the construction of livestock facilities. Inactivated bovine blood serum was used as a protective substrate (to simulate production conditions).

Results. On smooth construction materials, the biocidal effect of the new disinfectant against microflora isolates of various resistance groups was achieved using the following application modes: 1.0% for 180 min, 2.0% for 120 min, and 5.0% for 120 min at a consumption rate of 250 mL/m2. On concrete construction materials, the effect was achieved at: 2.0% for 180 min, 3.0% for 180 min, and 6.0% for 240 min after double treatment with a 120-minute interval at an consumption rate of 400 mL/m2.

Conclusion. Based on the results of the studies, it was established that the tested disinfectant possesses high bactericidal activity against microorganisms with low, medium, and high resistance to chemical disinfectants under conditions simulating production environment of livestock facilities. Analyzing the data obtained, it can be concluded that further experiments to study the biocidal effect of the new disinfectant in actual conditions of livestock facilities are expedient for developing effective decontamination protocols for agro-industrial production facilities.

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