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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">veterinary</journal-id><journal-title-group><journal-title xml:lang="ru">Ветеринария сегодня</journal-title><trans-title-group xml:lang="en"><trans-title>Veterinary Science Today</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2304-196X</issn><issn pub-type="epub">2658-6959</issn><publisher><publisher-name>"Veinard"</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.29326/2304-196X-2026-15-1-38-45</article-id><article-id custom-type="elpub" pub-id-type="custom">veterinary-979</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ОРИГИНАЛЬНЫЕ СТАТЬИ | ВЕТЕРИНАРНАЯ МИКРОБИОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ORIGINAL ARTICLES | VETERINARY MICROBIOLOGY</subject></subj-group></article-categories><title-group><article-title>Идентификация серотипов и анализ антибиотикорезистентности изолятов Listeria monocytogenes, выделенных из продукции животного происхождения за период с 2021 по 2024 г.</article-title><trans-title-group xml:lang="en"><trans-title>Serotype identification and antibiotic resistance analysis of Listeria monocytogenes isolates recovered from animal products in 2021–2024</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Акулич</surname><given-names>О. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Akulich</surname><given-names>O. А.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Акулич Ольга Андреевна, аспирант</p><p>ул. Гвардейская, 6, мкр. Юрьевец, г. Владимир, 600901</p></bio><bio xml:lang="en"><p>Olga А. Akulich, Postgraduate Student</p><p>ul. Gvardeyskaya, 6, Yur’evets, Vladimir 600901</p></bio><email xlink:type="simple">akulich.olgand@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7510-1269</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шадрова</surname><given-names>Н. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Shadrova</surname><given-names>N. B.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Шадрова Наталья Борисовна, канд. биол. наук, заведующий отделом микробиологических исследований</p><p>ул. Гвардейская, 6, мкр. Юрьевец, г. Владимир, 600901</p></bio><bio xml:lang="en"><p>Natalya B. Shadrova, Cand. Sci. (Biology), Head of Department for Microbiological Testing</p><p>ul. Gvardeyskaya, 6, Yur’evets, Vladimir 600901</p></bio><email xlink:type="simple">shadrova@arriah.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0714-3912</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Денисова</surname><given-names>Г. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Denisova</surname><given-names>G. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Денисова Галина Сергеевна, канд. биол. наук, руководитель Владимирской испытательной лаборатории</p><p>ул. Гвардейская, 6, мкр. Юрьевец, г. Владимир, 600901</p></bio><bio xml:lang="en"><p>Galina S. Denisova, Cand. Sci. (Biology), Head of the Vladimir Testing Centre</p><p>ul. Gvardeyskaya, 6, Yur’evets, Vladimir 600901</p></bio><email xlink:type="simple">skitovich@arriah.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБУ «Федеральный центр охраны здоровья животных» (ФГБУ «ВНИИЗЖ»)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal Centre for Animal Health</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2026</year></pub-date><pub-date pub-type="epub"><day>18</day><month>03</month><year>2026</year></pub-date><volume>15</volume><issue>1</issue><fpage>38</fpage><lpage>45</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Акулич О.А., Шадрова Н.Б., Денисова Г.С., 2026</copyright-statement><copyright-year>2026</copyright-year><copyright-holder xml:lang="ru">Акулич О.А., Шадрова Н.Б., Денисова Г.С.</copyright-holder><copyright-holder xml:lang="en">Akulich O.А., Shadrova N.B., Denisova G.S.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://veterinary.arriah.ru/jour/article/view/979">https://veterinary.arriah.ru/jour/article/view/979</self-uri><abstract><sec><title>Введение</title><p>Введение. Listeria monocytogenes – патогенный микроорганизм, вызывающий большое количество летальных исходов вследствие потребления контаминированной продукции животного происхождения. Этим обусловлена актуальность мониторинга распространения возбудителя листериоза в сырье, продукции животного происхождения и объектах окружающей среды, а также антибиотикорезистентности изолятов.</p></sec><sec><title>Цель исследования</title><p>Цель исследования. Идентификация серотипов и анализ антибиотикорезистентности изолятов Listeria monocytogenes, выделенных из продукции животного происхождения за период с 2021 по 2024 г.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Работа была выполнена на базе отдела микробиологических исследований Владимирской испытательной лаборатории ФГБУ «ВНИИЗ Ж». Изоляты бактерий рода Listeria идентифицировали с использованием метода времяпролетной масс-спектрометрии. Определение антибиотикорезистентности изолятов, относящихся к виду Listeria monocytogenes, проводили диско-диффузионным методом. Значения зон задержки роста интерпретировали согласно российским рекомендациям «Определение чувствительности микроорганизмов к антимикробным препаратам» (МАКМАХ, версия 2025-01). Серологические группы листерий были идентифицированы с использованием метода полимеразной цепной реакции в режиме реального времени с применением праймеров производства НПК «Синтол» (Россия).</p></sec><sec><title>Результаты</title><p>Результаты. В статье представлены результаты исследований антибиотикорезистентности 77 изолятов Listeria monocytogenes, выявленных в продукции животного происхождения в 2021–2024 гг., а также их дифференциации по серогруппам. Чаще всего Listeria monocytogenes определяли в продукции из мяса птицы. Выявленные изоляты имели максимальную резистентность к цефуроксиму, сульфаметоксазолу/триметоприму, норфлоксацину, рифампицину, левофлоксацину и канамицину. При этом большинство изолятов проявило устойчивость более чем к одному антимикробному препарату. В рамках исследования установлена принадлежность изолятов Listeria monocytogenes к следующим серогруппам: IIa (серотипы 1/2а, 3а) – 92,2%; IIc (серотипы 1/2с, 3с) – 5,2%; IVb (серотипы 4b, 4d, 4e) – 2,6%.</p></sec><sec><title>Заключение</title><p>Заключение. Показано распространение устойчивости, в том числе множественной, среди изолятов Listeria monocytogenes, выявленных в продукции животного происхождения в 2021–2024 гг. В результате проведенного исследования было определено присутствие листерий, относящихся к группе IVb (серотипы 4b, 4d, 4e). Однако доминирующая часть изолятов рода Listeria monocytogenes была классифицирована как группа IIa (серотипы 1/2а, 3а).</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Introduction</title><p>Introduction. Listeria monocytogenes is a pathogenic microorganism that causes a large number of deaths due to the consumption of contaminated animal products. This underpins the relevance of monitoring the spread of the listeriosis agent in raw materials, animal products, and environmental objects, as well as the antibiotic resistance of isolates.</p></sec><sec><title>Objective</title><p>Objective. Serotype identification and antibiotic resistance analysis of Listeria monocytogenes isolates recovered from animal products in 2021–2024.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. The work was performed at the Microbiology Unit of the Vladimir Testing Laboratory, the Federal Centre for Animal Health. Listeria genus bacteria isolates were identified using time-of-flight mass spectrometry. Antibiotic resistance of isolates belonging to the species Listeria monocytogenes was determined by the disk diffusion method. The values of the growth retardation zones were interpreted according to the Russian recommendations “Determination of the sensitivity of microorganisms to antimicrobial drugs” (IACMAC, version 2025-01). Listeria serological groups were identified using real-time polymerase chain reaction (qPCR) with primers manufactured by Syntol (Russia).</p></sec><sec><title>Results</title><p>Results. The article presents the results of antibiotic resistance testing of 77 Listeria monocytogenes isolates detected in animal products in 2021–2024, as well as their differentiation by serogroups. Listeria monocytogenes was most frequently detected in poultry products. The detected isolates showed maximum resistance to cefuroxime, sulfamethoxazole/trimethoprim, norfloxacin, rifampicin, levofloxacin, and kanamycin. Moreover, most isolates exhibited resistance to more than one antimicrobial medicinal product. The study established the belonging of the Listeria monocytogenes isolates to the following serogroups: IIa (serotypes 1/2a, 3a) – 92.2%; IIc (serotypes 1/2c, 3c) – 5.2%; IVb (serotypes 4b, 4d, 4e) – 2.6%.</p></sec><sec><title>Conclusion</title><p>Conclusion. The spread of resistance, including multidrug resistance, among Listeria monocytogenes isolates detected in animal products in 2021–2024 was demonstrated. The study identified the presence of listeria belonging to group IVb (serotypes 4b, 4d, 4e). However, the dominant part of Listeria monocytogenes isolates was classified as group IIa (serotypes 1/2a, 3a).</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>Listeria monocytogenes</kwd><kwd>антибиотикорезистентность</kwd><kwd>серотип</kwd><kwd>чувствительность к антимикробным препаратам</kwd><kwd>полимеразная цепная реакция в режиме реального времени</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Listeria monocytogenes</kwd><kwd>antibiotic resistance</kwd><kwd>serotype</kwd><kwd>antimicrobial susceptibility</kwd><kwd>real-time polymerase chain reaction</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена за счет средств ФГБУ «ВНИИЗЖ» в рамках тематики научно-исследовательских работ «Ветеринарное благополучие».</funding-statement><funding-statement xml:lang="en">The study was funded by the Federal Centre for Animal Health within the research topic “Veterinary Welfare”</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Ковалев В. 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