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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">veterinary</journal-id><journal-title-group><journal-title xml:lang="ru">Ветеринария сегодня</journal-title><trans-title-group xml:lang="en"><trans-title>Veterinary Science Today</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2304-196X</issn><issn pub-type="epub">2658-6959</issn><publisher><publisher-name>"Veinard"</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.29326/2304-196X-2025-14-4-418-425</article-id><article-id custom-type="elpub" pub-id-type="custom">veterinary-964</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ОРИГИНАЛЬНЫЕ СТАТЬИ | ВЕТЕРИНАРНАЯ МИКРОБИОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ORIGINAL ARTICLES | VETERINARY MICROBIOLOGY</subject></subj-group></article-categories><title-group><article-title>Индикация бактерий Listeria monocytogenes при оценке микробиологической контаминации сырья и продуктов животного происхождения</article-title><trans-title-group xml:lang="en"><trans-title>Detection of Listeria monocytogenes while testing food raw materials and products of animal origin for microbiological contamination</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0003-9445-0829</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Логацкая</surname><given-names>Л. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Logatskaya</surname><given-names>Lyubov N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Логацкая Любовь Николаевна, аспирант, заместитель руководителя, </p><p>пр. Гагарина, 97, г. Нижний Новгород, 603107.</p></bio><bio xml:lang="en"><p>Lyubov N. Logatskaya, Postgraduate Student, Deputy Head of Nizhny Novgorod Testing Laboratory,</p><p>pr. Gagarina, 97, Nizhny Novgorod 603107.</p></bio><email xlink:type="simple">logackaya@arriah.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-3143-7339</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Прунтова</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Pruntova</surname><given-names>Olga V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Прунтова Ольга Владиславовна, д-р биол. наук, профессор, главный научный сотрудник информационно-аналитического центра, </p><p>ул. Гвардейская, 6, мкр. Юрьевец, г. Владимир, 600901.</p></bio><bio xml:lang="en"><p>Olga V. Pruntova, Dr. Sci. (Biology), Professor, Chief Researcher, Information and Analysis Centre, </p><p>ul. Gvardeyskaya, 6, Yur’evets, Vladimir 600901.</p></bio><email xlink:type="simple">pruntova@arriah.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9857-5915</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Жбанова</surname><given-names>Т. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Zhbanova</surname><given-names>Tatyana V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Жбанова Татьяна Валентиновна, канд. биол. наук, младший научный сотрудник отдела образования и научной информации,</p><p>ул. Гвардейская, 6, мкр. Юрьевец, г. Владимир, 600901.</p></bio><bio xml:lang="en"><p>Tatyana V. Zhbanova, Cand. Sci. (Biology), Junior Researcher, Education and Scientific Support Department, </p><p>ul. Gvardeyskaya, 6, Yur’evets, Vladimir 600901.</p><p> </p></bio><email xlink:type="simple">zhbanova@arriah.ru</email><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Нижегородский филиал ФГБУ «Федеральный центр охраны здоровья животных» (Нижегородский филиал ФГБУ «ВНИИЗЖ»)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Nizhny Novgorod Branch of Federal Centre for Animal Health</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ФГБУ «Федеральный центр охраны здоровья животных» (ФГБУ «ВНИИЗЖ»)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal Centre for Animal Health</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>21</day><month>12</month><year>2025</year></pub-date><volume>14</volume><issue>4</issue><fpage>418</fpage><lpage>425</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Логацкая Л.Н., Прунтова О.В., Жбанова Т.В., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Логацкая Л.Н., Прунтова О.В., Жбанова Т.В.</copyright-holder><copyright-holder xml:lang="en">Logatskaya L.N., Pruntova O.V., Zhbanova T.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://veterinary.arriah.ru/jour/article/view/964">https://veterinary.arriah.ru/jour/article/view/964</self-uri><abstract><sec><title>Введение</title><p>Введение. Проблема контаминации пищевых продуктов листериями не теряет своей актуальности. Употребление в пищу обсемененных патогенными бактериями рода Listeria продуктов животного происхождения в сыром или недостаточно термически обработанном виде приводит к заражению человека. По данным Государственного доклада Роспотребнадзора, в 2023 г. в нашей стране было зарегистрировано 100 случаев листериоза, из которых 18 –  с летальным исходом. В последние годы наблюдается рост контаминации листериями пищевых продуктов как отечественного, так и зарубежного производства. Таким образом, выявление патогенных видов Listeria в продуктах животного происхождения, пищевом сырье и готовых пищевых продуктах является актуальной задачей.</p></sec><sec><title>Цель исследования</title><p>Цель исследования. Определение контаминации бактериями Listeria monocytogenes продуктов животного происхождения (мясных, рыбных, молочных), произведенных и реализуемых в Нижегородской области в период с 2023 по 2024 г.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Исследование проб, а также идентификацию чистой культуры микроорганизмов проводили в соответствии с ГОСТ 32031-2022 «Продукты пищевые. Методы выявления бактерий Listeria monocytogenes и других видов Listeria (Listeria spp.)».</p></sec><sec><title>Результаты</title><p>Результаты. При анализе и обобщении полученных данных было показано, что из 3650 исследованных проб бактериями L. monocytogenes были контаминированы 57 образцов (1,6%). В таких категориях продуктов, как полуфабрикаты мясные смешанного состава, продукты из говядины и мяса птицы, было определено наибольшее количество контаминированных проб. При исследовании проб рыбных продуктов инцидентность L. monocytogenes составила 1,1%. Наибольший уровень контаминации отмечен в таких видах продуктов, как фарш говяжий (10,7%), полуфабрикаты из мяса птицы в тестовой оболочке (9,3%), мясо птицы механической обвалки (7,1%), полуфабрикаты крупнокусковые (4,6%) и субпродукты (4,3%) из говядины, полуфабрикаты из мяса птицы рубленые (4,2%).</p></sec><sec><title>Заключение</title><p>Заключение. В результате испытаний было установлено, что количество контаминированных проб полуфабрикатов мясных смешанного состава достигло 4,3%, несоответствия требованиям безопасности продуктов из говядины выявлены в 3,7% случаев, 2,8% проб продуктов птицеводства были обсеменены бактериями L. monocytogenes. Количество и процентное соотношение контаминированных проб замороженных и охлажденных продуктов достоверно не различались и составили 0,7 и 0,8% соответственно. Бактерии L. monocytogenes не были выявлены в пробах молочных и готовых мясных продуктов, не требующих термической обработки.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Introduction</title><p>Introduction. Listeria-contaminated food remains an ongoing concern. Consumption of raw or undercooked animal-derived products contaminated with pathogenic Listeria results in human infection. The Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor) documented 100 listeriosis cases in 2023, with 18 cases resulting in death. In recent years, there has been an increase in Listeria contamination of both domestically produced and imported food products. Thus, detection of pathogenic Listeria in the products of animal origin, food raw materials, and ready-to-eat products remains a critical task.</p></sec><sec><title>Objective</title><p>Objective. Detecting Listeria monocytogenes contamination in products of animal origin (meat, fish, dairy) manufactured and marketed in the Nizhny Novgorod Oblast from 2023 to 2024.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. The samples were analysed and pure microbial cultures were identified in accordance with GOST 32031-2022 “Food products. Methods for detection of Listeria monocytogenes and other Listeria (Listeria spp.)”.</p></sec><sec><title>Results</title><p>Results. Analysis and synthesis of the obtained data revealed that out of 3,650 tested samples, 57 (1.6%) were contaminated with L. monocytogenes bacteria. The highest number of contaminated samples was found among such product categories as combined semi-finished meat products, beef products, and poultry meat products. The incidence of L. monocytogenes in samples of fishery products was 1.1%. The highest levels of contamination were detected in the following products: minced beef (10.7%), poultry meat products wrapped in dough (9.3%), mechanically deboned poultry meat (7.1%), large-cut semi-finished products (4.6%), beef offal (4.3%), and chopped semi-finished poultry meat products (4.2%).</p></sec><sec><title>Conclusion</title><p>Conclusion. The test results show that the number of contaminated samples among combined semi-finished meat products was 4.3%, non-compliance with the safety requirements of beef products was detected in 3.7%; 2.8% of poultry product samples were contaminated with L. monocytogenes bacteria. The number and percentage of contaminated samples among frozen and refrigerated products did not significantly differ and amounted to 0.7 and 0.8%, respectively.  L. monocytogenes were not detected in samples of dairy and ready-to-eat meat products that do not require heat treatment.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>листериоз</kwd><kwd>Listeria monocytogenes</kwd><kwd>контаминация</kwd><kwd>мясо</kwd><kwd>молоко</kwd><kwd>пищевые продукты</kwd></kwd-group><kwd-group xml:lang="en"><kwd>listeriosis</kwd><kwd>Listeria monocytogenes</kwd><kwd>contamination</kwd><kwd>meat</kwd><kwd>milk</kwd><kwd>food products</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена за счет средств ФГБУ «ВНИИЗЖ» в рамках тематики научно-исследовательских работ «Ветеринарное благополучие».</funding-statement><funding-statement xml:lang="en">The study was conducted within the state assignment “Detection of animal transboundary disease pathogens, study of their biological properties, and investigation of the entry and spread patterns of diseases caused by these pathogens”.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Фофанова Т. 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