veterinaryВетеринария сегодняVeterinary Science Today2304-196X2658-6959"Veinard"10.29326/2304-196X-2024-13-3-248-254veterinary-845Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ | БОЛЕЗНИ ПТИЦORIGINAL ARTICLES | AVIAN DISEASESПрименение вакцины «ВНИИЗЖ-АвиФлуВак» для индеек, гусей и утокUse of “ARRIAH-AviFluVac” vaccine in turkeys, geese and duckshttps://orcid.org/0000-0002-9672-8594МорозН. В.MorozN. V.

Мороз Наталья Владимировна - канд. вет. наук, заведующий лабораторией профилактики болезней птиц 

мкр. Юрьевец, г. Владимир, 600901

Natalia  V.  Moroz - Cand. Sci. (Veterinary Medicine), Head of Laboratory for Avian Diseases Prevention

Yur’evets, Vladimir 600901

moroz@arriah.ruhttps://orcid.org/0000-0001-6802-9940ФроловС. В.FrolovS. V.

Фролов Сергей Владимирович - канд. вет. наук, начальник отдела профилактики болезней птиц 

мкр. Юрьевец, г. Владимир, 600901

Sergey  V.  Frolov - Cand. Sci. (Veterinary Medicine), Head of Department for Avian Disease Prevention

Yur’evets, Vladimir 600901

frolov@arriah.ruhhttps://orcid.org/0000-0001-7489-1772ИрзаВ. Н.IrzaV. N.

Ирза Виктор Николаевич - д-р вет. наук, доцент, главный научный сотрудник информационно-аналитического центра 

мкр. Юрьевец, г. Владимир, 600901

Viktor N. Irza - Dr. Sci. (Veterinary Medicine), Associate Professor, Chief Researcher, Information and Analysis Centre

Yur’evets, Vladimir 600901

irza@arriah.ruhttps://orcid.org/0000-0001-5434-6179ЩербаковаЛ. О.ScherbakovaL. O.

Щербакова Лидия Олеговна - канд. биол. наук, ведущий научный сотрудник референтной лаборатории вирусных болезней птиц

мкр. Юрьевец, г. Владимир, 600901

Lidia O. Scherbakova - Cand. Sci. (Biology), Leading Researcher, Reference Laboratory for Avian Viral Diseases

Yur’evets, Vladimir 600901

scherbakova@arriah.ruhttps://orcid.org/0000-0002-1898-4576КулаковВ. Ю.KulakovV. Yu.

Кулаков Владимир Юрьевич - канд. вет. наук, ведущий научный сотрудник лаборатории профилактики болезней птиц 

мкр. Юрьевец, г. Владимир, 600901

Vladimir Yu. Kulakov - Cand. Sci. (Veterinary Medicine), Leading Researcher, Laboratory for Avian Diseases Prevention

Yur’evets, Vladimir 600901

kulakov@arriah.ruФГБУ «Федеральный центр охраны здоровья животных» (ФГБУ «ВНИИЗЖ»)РоссияFederal Centre for Animal HealthRussian Federation202416092024133248254Copyright © Мороз Н.В., Фролов С.В., Ирза В.Н., Щербакова Л.О., Кулаков В.Ю., 20242024Мороз Н.В., Фролов С.В., Ирза В.Н., Щербакова Л.О., Кулаков В.Ю.Moroz N.V., Frolov S.V., Irza V.N., Scherbakova L.O., Kulakov V.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://veterinary.arriah.ru/jour/article/view/845

В лабораторных и производственных условиях показано, что вакцина против гриппа птиц Н5 «ВНИИЗЖ-АвиФлуВак» является эффективным препаратом для профилактики болезни у гусей, уток и индеек. Вакцина при введении уткам в дозах 0,5; 1,0 и 1,5 см3 со 100%-й эффективностью защищала птиц от заболевания и гибели при заражении актуальным вирусом высокопатогенного гриппа птиц подтипа H5N1 генетической клады 2.3.4.4b. Однократная прививка в дозе от 0,5 до 1,5 см3 вызывала образование антител, выявляемых в реакции торможения гемагглютинации, в титрах от 4,3 до 6,1 log2. Вакцина способствовала снижению выделения вирулентного вируса утками в 9–26 раз. Протективная защита индеек, привитых в дозе 1,0 см3, обеспечивалась на уровне 87,5% при заражении вирусом высокопатогенного гриппа птиц подтипа H5N1 клады 2.3.4.4b. Вакцина вызывала образование антител в титрах 4,9 и 5,5 log2 у индеек при однократном и двукратном введении в дозе 1,0 см3 соответственно. Установлено, что при двукратном применении препарата «ВНИИЗЖ-АвиФлуВак» в дозе 1,0 см3 уровень поствакцинальных антител к вирусу гриппа птиц был выше 5,0 log2 у 75,9–90,0% популяции гусей. Рациональным решением использования вакцины для индеек, уток и гусей является ее применение в двойной коммерческой дозе и как минимум двукратное введение. Также была установлена более высокая видовая устойчивость уток к заражению вирусом гриппа птиц подтипа Н5 клады 2.3.4.4b по сравнению с индейками

“ARRIAH-AviFluVac” vaccine against H5 avian influenza was demonstrated to be effective for  ducks, geese and turkeys both in the laboratory and production environment. When administered to ducks at 0.5; 1.0 and 1.5 cm3, the vaccine provided 100%-effective protection of birds against the disease and death after challenge with the relevant high pathogenicity avian influenza virus of subtype H5N1, clade 2.3.4.4b. Singular 0.5–1.5 cm3 inoculation induced formation of antibodies, which were detected in the hemagglutination inhibition test at the titres that ranged from 4.3 to 6.1 log2. The vaccine facilitated 9–26-fold decrease in the virulent virus shedding by the ducks. Protection of turkeys vaccinated at the dose of 1.0 cm3 was maintained at the level of 87.5% after challenge with high pathogenicity avian influenza virus of subtype H5N1, clade 2.3.4.4b. The vaccine induced formation of antibodies at the titres of 4.9 and 5.5 log2 in turkeys after singular and double vaccination at the dose of 1.0 cm3, respectively. It was demonstrated, that after double administration of 1.0 cm3 of “ARRIAH-AviFluVac” vaccine, the post-vaccinal avian influenza antibody level exceeded 5.0 log2 in 75.9–90.0% of the geese population. The most appropriate way of the vaccine use in turkeys, ducks and geese involves at least its double administration at the double commercial dose. Higher species resistance of ducks to the challenge with avian influenza virus of subtype H5, clade 2.3.4.4bas compared to turkeys was also demonstrated.

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The authors declare that there are no conflicts of interest present.