veterinaryВетеринария сегодняVeterinary Science Today2304-196X2658-6959"Veinard"10.29326/2304-196X-2024-13-1-44-56veterinary-789Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ | ЯЩУРORIGINAL ARTICLES | FOOT-AND-MOUTH DISEASEИзучение серотипоспецифичности диагностических тест-систем для выявления антител к структурным белкам вируса ящура иммуноферментным анализомTesting of diagnostic test-systems for detection of antibodies to foot-and-mouth disease virus structural proteins with enzyme-linked immunosorbent assay for their serotype specificityhttps://orcid.org/0009-0001-0029-6345ЛуговскаяН. Н.LugovskayaN. N.

Луговская Наталия Николаевна, канд. биол. наук, заведующий сектором лаборатории профилактики ящура

мкр. Юрьевец, г. Владимир, 600901

Natalia N. Lugovskaya, Cand. Sci. (Biology), Head of Sector, Laboratory for FMD Prevention

Yur’evets, Vladimir 600901

lugovskaya@arriah.ruhttps://orcid.org/0009-0007-5877-178XСилантьеваЕ. А.SilantevaE. A.

Силантьева Екатерина Андреевна, аспирант, ветеринарный врач лаборатории профилактики ящура

мкр. Юрьевец, г. Владимир, 600901

Ekaterina A. Silanteva, Postgraduate Student, Veterinarian, Laboratory for FMD Prevention

Yur’evets, Vladimir 600901

silantyeva_ea@arriah.ruhttps://orcid.org/0009-0002-8403-8665ОковытаяТ. В.OkovytayaT. V.

Оковытая Татьяна Владимировна, канд. биол. наук, старший научный сотрудник лаборатории  профилактики ящура

мкр. Юрьевец, г. Владимир, 600901

Tatyana V. Okovytaya, Cand. Sci. (Biology), Senior Researcher, Laboratory for FMD Prevention

Yur’evets, Vladimir 600901

okovitaya@arriah.ruХаритоноваА. А.KharitonovaA. A.

Харитонова Анастасия Александровна, ветеринарный врач лаборатории профилактики ящура

мкр. Юрьевец, г. Владимир, 600901

Anastasiya A. Kharitonova, Veterinarian, Laboratory for FMD Prevention

Yur’evets, Vladimir 600901

haritonova_aa@arriah.ruhttps://orcid.org/0000-0002-2508-8411ГочмурадовЫ. М.GochmuradovY. M.

Гочмурадов Ыхлас Мурадович, аспирант, ветеринарный врач лаборатории профилактики ящура

мкр. Юрьевец, г. Владимир, 600901

Ykhlas M. Gochmuradov, Postgraduate Student, Veterinarian, Laboratory for FMD Prevention

Yur’evets, Vladimir 600901

gochmuradov@arriah.ruРазгуляеваЕ. А.RazgulyaevaE. A.

Разгуляева Евгения Александровна, главный специалист лаборатории профилактики ящура

мкр. Юрьевец, г. Владимир, 600901

Evgeniya A. Razgulyaeva, Chief Specialist, Laboratory for FMD Prevention

Yur’evets, Vladimir 600901

БудинаО. О.BudinaO. O.

Будина Олеся Олеговна, главный специалист лаборатории профилактики ящура

мкр. Юрьевец, г. Владимир, 600901

Olesya O. Budina, Chief Specialist, Laboratory for FMD Prevention

Yur’evets, Vladimir 600901

budina@arriah.ruhttps://orcid.org/0009-0004-1556-5538ЯсневаЕ. А.YasnevaE. A.

Яснева Елена Анатольевна, канд. вет. наук, руководитель испытательной лаборатории ветеринарных препаратов

мкр. Юрьевец, г. Владимир, 600901

Elena A. Yasneva, Cand. Sci. (Veterinary Medicine), Head of the VMP Testing Laboratory

Yur’evets, Vladimir 600901

jasneva@arriah.ruФГБУ «Федеральный центр охраны здоровья животных» (ФГБУ «ВНИИЗЖ»)РоссияFederal Centre for Animal HealthRussian Federation2024150320241314456Copyright © Луговская Н.Н., Силантьева Е.А., Оковытая Т.В., Харитонова А.А., Гочмурадов Ы.М., Разгуляева Е.А., Будина О.О., Яснева Е.А., 20242024Луговская Н.Н., Силантьева Е.А., Оковытая Т.В., Харитонова А.А., Гочмурадов Ы.М., Разгуляева Е.А., Будина О.О., Яснева Е.А.Lugovskaya N.N., Silanteva E.A., Okovytaya T.V., Kharitonova A.A., Gochmuradov Y.M., Razgulyaeva E.A., Budina O.O., Yasneva E.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://veterinary.arriah.ru/jour/article/view/789

При изучении серотипоспецифичности 24 диагностических иммуноферментных тест-систем для определения антител к структурным белкам вируса ящура 6 производителей (ФГБУ «ВНИИЗЖ», Prionics, IZSLER, Innovative Diagnostics, BIONOTE и MEDIAN Diagnostics) было исследовано 138 образцов сыворотки крови свиней и крупного рогатого скота, вакцинированных против ящура одного или двух серотипов либо инфицированных вирусом ящура. Все использованные тест-системы наряду с ярко выраженной серотипоспецифической активностью (гомологичная реакция) выявляли также некоторое количество перекрестно-реагирующих вирусоспецифических антител, что было обусловлено рядом причин, связанных с консервативными эпитопами в аминокислотной последовательности капсидных полипептидов VP1–VP3 вириона возбудителя ящура, с доступностью внутренних консервативных эпитопов полипептида VP4 для иммунной системы животного при репликации вируса или деструкции вакцинного антигена (вируса) в организме живот­ного в ходе иммунного процесса, а также с компонентным составом экспериментальной противоящурной вакцины и др. Тем не менее анализ большого массива данных (около 3500 исследований) показал, что гомологичная серотипоспецифическая реакция в целом была значительно выше и являлась доминирующей, доля вирусоспецифических незащитных антител, в том числе и перекрестно-реагирующих, была не столь значительной и не искажала результаты оценки иммуногенности противоящурной вакцины в иммуноферментном анализе. В сомнительных случаях требуется подтверждение результатов в других серологических реакциях. Оптимальным вариантом следует считать комплексные исследования на ящур с привлечением разных методов диагностики, таких как иммуноферментный анализ с использованием стандартных и референтных тест-систем и/или реакция вирусной ней­трализации в культуре клеток.

A total of 138 serum samples from pigs and cattle vaccinated against foot-and-mouth disease virus (FMDV) of one or two serotypes or infected with FMDV were used for testing of 24 enzyme-linked immunosorbent assay (ELISA) diagnostic tests-systems for detection of antibodies against FMDV structural proteins produced by 6 manufacturers (Federal Centre for Animal Health, Prionics, IZSLER, Innovative Diagnostics, BIONOTE and MEDIAN Diagnostics) for their serotype-specificity. All used test-systems detected apparent serotype-specific activity (homologous reaction) as well as cross-reacting virus-specific antibodies that was accounted for some reasons related to conservative epitopes in amino acid sequence of FMDV virion capsid VP1–VP3 polypeptides, accessibility of internal conservative epitopes of VP4 polypeptide for the animal’s immune system during virus replication or vaccine antigen (virus) destruction in the animal’s body in the process of immunity development, as well as the pilot anti-FMD vaccine composition, etc. Nevertheless, the analysis of a large data set (about 3,500 tests) showed that the homologous serotype-specific reaction in general was significantly higher and predominant, the proportion of virus-specific non-protective antibodies, including cross-reacting ones, was not significant and did not distort the results of ELISA tests of anti-FMD vaccine for its immunogenicity. Inconclusive test results require confirmation with other serological tests. Complex tests for FMDV using different diagnostic methods such as ELISA with standard and reference test-systems and/or virus neutralization test in cell culture are to be considered as the best option.

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The authors declare that there are no conflicts of interest present.