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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">veterinary</journal-id><journal-title-group><journal-title xml:lang="ru">Ветеринария сегодня</journal-title><trans-title-group xml:lang="en"><trans-title>Veterinary Science Today</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2304-196X</issn><issn pub-type="epub">2658-6959</issn><publisher><publisher-name>"Veinard"</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.29326/2304-196X-2022-11-2-142-148</article-id><article-id custom-type="elpub" pub-id-type="custom">veterinary-642</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ОРИГИНАЛЬНЫЕ СТАТЬИ | БОЛЕЗНИ ПТИЦ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ORIGINAL ARTICLES | AVIAN DISEASES</subject></subj-group></article-categories><title-group><article-title>Серологический мониторинг гриппа птиц и ньюкаслской болезни на территории Российской Федерации в 2020 г.</article-title><trans-title-group xml:lang="en"><trans-title>Serological monitoring of avian influenza and Newcastle disease in the Russian Federation in 2020</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-6959-3965</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кулагина</surname><given-names>М. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Kulagina</surname><given-names>M. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Кулагина Мария Александровна - кандидат биологических наук, научный сотрудник референтной лаборатории вирусных болезней птиц ВНИИЗЖ.</p><p>600901, Владимир,  мкр. Юрьевец.</p></bio><bio xml:lang="en"><p>Maria A. Kulagina - Candidate of Science (Biology), Researcher, Reference Laboratory for Avian Viral Diseases, ARRIAH.</p><p>600901, Vladimir, Yur’evets.</p></bio><email xlink:type="simple">kulagina@arriah.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-7674-639X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Волкова</surname><given-names>М. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Volkova</surname><given-names>M. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Волкова  Марина Алексеевна - кандидат  биологических наук, ведущий научный сотрудник референтной лаборатории вирусных болезней птиц ВНИИЗЖ.</p><p>Владимир.</p></bio><bio xml:lang="en"><p>Marina A. Volkova - Candidate  of Science  (Biology), Leading Researcher,  Reference  Laboratory  for  Avian  Viral  Diseases, ARRIAH.</p><p>Vladimir.</p></bio><email xlink:type="simple">volkovama@arriah.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5846-1262</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чвала</surname><given-names>Ир. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Chvala</surname><given-names>Ir. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Чвала Ирина Александровна - кандидат  биологических наук, ведущий научный сотрудник референтной лаборатории вирусных болезней птиц ВНИИЗЖ.</p><p>Владимир.</p></bio><bio xml:lang="en"><p>Irina A. Chvala - Candidate  of  Science  (Biology),  Leading Researcher,  Reference  Laboratory  for  Avian  Viral  Diseases, ARRIAH.</p><p>Vladimir.</p></bio><email xlink:type="simple">chvala_ia@arriah.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3176-157X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Осипова</surname><given-names>О. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Osipova</surname><given-names>O. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Осипова Ольга Сергеевна - ветеринарный врач референтной лаборатории   вирусных  болезней  птиц ВНИИЗЖ.</p><p>Владимир.</p></bio><bio xml:lang="en"><p>Olga S. Osipova - Veterinarian, Reference Laboratory for Avian Viral Diseases, ARRIAH.</p><p>Vladimir.</p></bio><email xlink:type="simple">osipova@arriah.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0383-9912</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ярославцева</surname><given-names>П. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Yaroslavtseva</surname><given-names>P. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ярославцева Полина  Сергеевна - кандидат биологических наук, научный сотрудник референтной лаборатории вирусных болезней птиц ВНИИЗЖ.</p><p>Владимир.</p></bio><bio xml:lang="en"><p>Polina S. Yaroslavtseva - Candidate  of  Science  (Biology), Researcher,  Reference  Laboratory  for  Avian  Viral  Diseases, ARRIAH.</p><p>Vladimir.</p></bio><email xlink:type="simple">yaroslavtseva@arriah.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1681-5795</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Андрейчук</surname><given-names>Д. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Andreychuk</surname><given-names>D. B.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Андрейчук Дмитрий Борисович - кандидат биологических наук, заведующий референтной лабораторией вирусных болезней птиц ВНИИЗЖ.</p><p>Владимир.</p></bio><bio xml:lang="en"><p>Dmitry B. Andreychuk - Candidate  of Science (Biology), Head of Reference Laboratory for Avian Viral Diseases, ARRIAH.</p><p>Vladimir.</p></bio><email xlink:type="simple">andreychuk@arriah.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1659-3256</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чвала</surname><given-names>И. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Chvala</surname><given-names>I. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Чвала Илья Александрович - кандидат ветеринарных  наук, заместитель директора по НИР ВНИИЗЖ.</p><p>Владимир.</p></bio><bio xml:lang="en"><p>Ilya A. Chvala - Candidate  of Science  (Veterinary Medicine), Deputy Director for Research, ARRIAH.</p><p>Vladimir.</p></bio><email xlink:type="simple">chvala@arriah.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Федеральный центр охраны здоровья животных</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal Centre for Animal Health</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>10</day><month>06</month><year>2022</year></pub-date><volume>11</volume><issue>2</issue><fpage>142</fpage><lpage>148</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Кулагина М.А., Волкова М.А., Чвала И.А., Осипова О.С., Ярославцева П.С., Андрейчук Д.Б., Чвала И.А., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Кулагина М.А., Волкова М.А., Чвала И.А., Осипова О.С., Ярославцева П.С., Андрейчук Д.Б., Чвала И.А.</copyright-holder><copyright-holder xml:lang="en">Kulagina M.A., Volkova M.A., Chvala I.A., Osipova O.S., Yaroslavtseva P.S., Andreychuk D.B., Chvala I.A.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://veterinary.arriah.ru/jour/article/view/642">https://veterinary.arriah.ru/jour/article/view/642</self-uri><abstract><p>В рамках реализации мероприятий Россельхознадзора с целью осуществления контроля за особо опасными болезнями и для своевременной выработки рекомендаций  по профилактике и борьбе с ними в референтную лабораторию вирусных болезней птиц ФГБУ «ВНИИЗЖ»  в течение 2020 г. было доставлено 36 986 проб сыворотки крови для исследования  на наличие антител к вирусу гриппа птиц и 30 325 проб – на наличие антител к вирусу ньюкаслской болезни. Пробы были отобраны от домашних, диких и синантропных птиц из 60 субъектов Российской Федерации. В результате лабораторных исследований антитела к вирусу гриппа типа А были обнаружены у вакцинированных кур из двух птицеводческих предприятий Приморского края. При типировании проб сыворотки крови в реакции торможения гемагглютинации установили, что выявленные антитела соответствовали вакцинному антигену по подтипу гемагглютинина  (А/Н9). Антитела к вирусу гриппа птиц подтипа Н9 были обнаружены в сыворотках крови от невакцинированных гусей с двух птицефабрик  Курганской области и одной птицефабрики Республики Башкортостан. В личных подсобных хозяйствах граждан, где проводится плановая вакцинопрофилактика гриппа птиц А/Н5, низкий уровень иммунитета установлен в Республике Адыгея и Чеченской Республике (0 и 15% соответственно) и высокий уровень – в Ростовской области (74%). Высокий уровень  антител к вирусу ньюкаслской  болезни  был установлен  у взрослой птицы в промышленных хозяйствах закрытого типа, что связано с массовой вакцинацией против данного заболевания. У цыплят-бройлеров отмечали наличие поствакцинальных антител в среднем в 44% исследованных проб сыворотки крови. Выявленные антитела к вирусам ньюкаслской болезни и гриппу птиц подтипа Н5 среди диких и синантропных птиц свидетельствуют  о циркуляции  данных вирусов на территории  Российской Федерации. Недостаточный уровень поствакцинальных антител указывает на сохранение риска возникновения эпизоотий среди домашних птиц промышленных птицеводческих предприятий и личных подсобных хозяйств.</p></abstract><trans-abstract xml:lang="en"><p>Within the framework of the Rosselkhoznadzor measures aimed at control of highly dangerous  diseases and development of timely recommendations for disease prevention and control, 36,986 serum samples to be tested for the presence of avian influenza virus antibodies and 30,325 serum samples to be tested for the presence of Newcastle disease virus antibodies were submitted to the FGBI “ARRIAH” Reference Laboratory for Avian Viral Diseases  in 2020. The samples were collected from domestic, wild and synanthropic birds in 60 Subjects of the Russian Federation. As a result of the laboratory diagnosis, antibodies against type A influenza virus were found in vaccinated chickens from two poultry farms in the Primorsky Krai. Typing of sample sera using hemagglutination inhibition test showed that the detected antibodies were specific to the haemagglutinin subtype of the vaccine antigen  (A/H9). Antibodies to the H9 subtype  avian influenza  virus were detected in sera of non-vaccinated geese from two poultry farms in the Kurgan Oblast and from one poultry farm in the Republic of Bashkortostan. As for the  backyards where  scheduled  vaccination  against  avian influenza  A/H5 is carried out, a low level of immunity  was seen in the Republics of Adygea and Chechnya (0 and 15%, respectively), while a high immunity level was observed in the Rostov Oblast (74%). High seroprevalence  of Newcastle disease virus was found in adult poultry in indoor industrial farms, which was associated with mass vaccination against the disease. In broiler chickens, post-vaccination antibodies were observed, on average, in 44% of the tested sera samples. The antibodies  against  Newcastle  disease  virus and avian influenza  virus subtype  H5 detected in wild and synanthropic birds indicate the circulation of these viruses in the Russian Federation. The insufficient level of post-vaccination antibodies suggests that the risk of epidemic among poultry in industrial poultry farms and backyards remains.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>мониторинг</kwd><kwd>грипп птиц</kwd><kwd>ньюкаслская болезнь</kwd><kwd>сыворотки крови</kwd><kwd>антитела</kwd><kwd>реакция торможения гемагглютинации</kwd><kwd>иммуноферментный анализ</kwd></kwd-group><kwd-group xml:lang="en"><kwd>monitoring</kwd><kwd>avian influenza</kwd><kwd>Newcastle disease</kwd><kwd>blood sera</kwd><kwd>antibodies</kwd><kwd>hemagglutination  inhibition test</kwd><kwd>ELISA</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена за счет бюджетных средств при проведении государственного эпизоотологического мониторинга.</funding-statement><funding-statement xml:lang="en">The study was financed from the budget  within the framework of the State epidemic monitoring.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Alexander D. 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