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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">veterinary</journal-id><journal-title-group><journal-title xml:lang="ru">Ветеринария сегодня</journal-title><trans-title-group xml:lang="en"><trans-title>Veterinary Science Today</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2304-196X</issn><issn pub-type="epub">2658-6959</issn><publisher><publisher-name>"Veinard"</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.29326/2304-196X-2021-1-36-59-67</article-id><article-id custom-type="elpub" pub-id-type="custom">veterinary-545</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ВЕТЕРИНАРНАЯ МИКРОБИОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>VETERINARY MICROBIOLOGY</subject></subj-group></article-categories><title-group><article-title>Индикация биопленок микроорганизмов при болезнях органов пищеварения ягнят</article-title><trans-title-group xml:lang="en"><trans-title>Visualization of microbial biofilms in case of digestive disorders in lambs</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2576-2020</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ленченко</surname><given-names>Е. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Lenchenko</surname><given-names>E. M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ленченко Екатерина Михайловна, доктор ветеринарных наук, профессор кафедры ветеринарной медицины</p><p>г. Москва</p></bio><bio xml:lang="en"><p>Ekaterina M. Lenchenko, Doctor of Science (Veterinary Medicine), Professor, Department of Veterinary Medicine</p><p>Moscow</p></bio><email xlink:type="simple">lenchenko-ekaterina@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1100-929X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сачивкина</surname><given-names>Н. П.</given-names></name><name name-style="western" xml:lang="en"><surname>Sachivkina</surname><given-names>N. P.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Сачивкина Надежда Павловна, кандидат биологических наук, доцент кафедры микробиологии и вирусологии медицинского института</p><p>г. Москва</p></bio><bio xml:lang="en"><p>Nadezda P. Sachivkina, Candidate of Science (Biology), Associate Professor, Department of Microbiology and Virology, Medical Institute</p><p>Moscow</p></bio><email xlink:type="simple">sachivkina@yandex.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-4724-6457</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Блюменкранц</surname><given-names>Д. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Blumenkrants</surname><given-names>D. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Блюменкранц Дмитрий Алексеевич, аспирант кафедры ветеринарной медицины</p><p>г. Москва</p></bio><bio xml:lang="en"><p>Dmitry А. Blumenkrants, Post-Graduate Student, Department of Veterinary Medicine</p><p>Moscow</p></bio><email xlink:type="simple">blumenkrants@icloud.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8199-3045</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Арсенюк</surname><given-names>Ю. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Arsenyuk</surname><given-names>A. Yu.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Арсенюк Анна Юрьевна, кандидат биологических наук, старший научный сотрудник отдела санитарной и клинической микробиологии</p><p>г. Москва</p></bio><bio xml:lang="en"><p>Anna Yu. Arsenyuk, Candidate of Science (Biology), Senior Researcher, Department of Sanitary and Clinical Microbiology</p><p>Moscow</p></bio><email xlink:type="simple">aarsenuk@gmail.com</email><xref ref-type="aff" rid="aff-3"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБОУ ВО «Московский государственный университет пищевых производств» (ФГБОУ ВО «МГУПП»)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Moscow State University of Food Production (FGBOU VO MGUPP)</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ФГАОУ ВО «Российский университет дружбы народов» (РУДН)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Peoples’ Friendship University of Russia (RUDN University)</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>ФГБУ «Всероссийский государственный Центр качества и стандартизации лекарственных средств для животных и кормов» (ФГБУ «ВГНКИ»)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>FGBI “The Russian State Centre for Animal Feed and Drug Standardization and Quality” (FGBI “VGNKI”)</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2021</year></pub-date><pub-date pub-type="epub"><day>29</day><month>03</month><year>2021</year></pub-date><volume>1</volume><issue>1</issue><fpage>59</fpage><lpage>67</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Ленченко Е.М., Сачивкина Н.П., Блюменкранц Д.А., Арсенюк Ю.А., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Ленченко Е.М., Сачивкина Н.П., Блюменкранц Д.А., Арсенюк Ю.А.</copyright-holder><copyright-holder xml:lang="en">Lenchenko E.M., Sachivkina N.P., Blumenkrants D.A., Arsenyuk A.Y.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://veterinary.arriah.ru/jour/article/view/545">https://veterinary.arriah.ru/jour/article/view/545</self-uri><abstract><p>Приведены результаты морфометрических и денситометрических показателей биопленок микроорганизмов, выделенных при болезнях органов пищеварения ягнят. Установлены изменения количественного и видового состава микробиоценозов кишечника при болезнях органов пищеварения по сравнению с клинически здоровыми ягнятами. При проведении исследований методами оптической микроскопии выявили формирование трехмерной структуры биопленок в виде плотной сети, состоящей из грамотрицательных и грамположительных бактерий, дрожжевых клеток, гифальных и псевдогифальных форм, окруженных межклеточным полимерным матриксом. Наличие бластоспор обусловливало увеличение количества адгезированных к субстрату клеток, происходило формирование биологической пленки, состоящей из палочковидных и округлых клеток, удерживающихся на клетках микроскопических грибов. В процессе дисперсии при разрушении межклеточного матрикса и отделении бактериальных и дрожжевых клеток от микроколоний обособлялись разветвленные структуры и колонизировали свободные от микроорганизмов участки субстрата. Оценку интенсивности формирования биопленок изучаемыми микроорганизмами проводили, измеряя оптическую плотность через 48 ч культивирования. Результаты люминесцентных микроскопических исследований показали, что динамика изменений жизнеспособных структур микроорганизмов характеризовалась чередующимися периодами снижения и увеличения интенсивности формирования биопленки. В исследуемых культурах микроорганизмов выявляли клетки, характеризующиеся способностью к активному росту и размножению, образующие сменяющие друг друга субпопуляции. При детекции жизнеспособности микроорганизмов в составе биопленок дифференцировали жизнеспособные (зеленый спектр люминесценции) и нежизнеспособные клетки (красный спектр люминесценции).</p></abstract><trans-abstract xml:lang="en"><p>The paper demonstrates morphometric and densitometric parameters of microbial biofilms recovered from lambs with digestive disorders. Changes of quantitative and species composition of the intestinal microbiocenoses in the lambs with digestive disorders were compared with the ones of the clinically healthy lambs. Light microscopy results demonstrated formation of three-dimensional biofilm structure in the form of dense grid consisting of gram-negative and gram-positive bacteria, yeast cells, hyphas and pseudohyphas surrounded with intracellular polymer matrix. Presence of blastospores aided to the increased number of cells attached to the substrate, and biofilm was formed, which consisted of rod and round cells attached to the microfungi cells. In the process of dispersion that occurred during the destruction of the intercellular matrix and bacterial and yeast cell detachment, branched structures separated from the microcolonies and colonized microorganism- free regions of the substrate. The intensity of biofilm formation by the microorganisms under study was evaluated by optic density measurement in 48 hours of cultivation. Fluorescence microscopy results demonstrated that the dynamics of changes of the viable microbial structures was specified by intermittent periods of increased or decreased biofilm formation intensity. Cells characterized by active growth and replication and forming alternating subpopulations were detected in the examined microbial cultures. When determining the viability of the microorganisms in the biofilms, the viable (green fluorescence) and non-viable (red fluorescence) cells were differentiated.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>Адгезия</kwd><kwd>биопленки</kwd><kwd>бактерии</kwd><kwd>дисперсия</kwd><kwd>микроскопические грибы</kwd><kwd>колонизационная резистентность</kwd><kwd>межклеточный матрикс</kwd><kwd>люминесценция</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Attachment</kwd><kwd>biofilms</kwd><kwd>bacteria</kwd><kwd>dispersion</kwd><kwd>microfungi</kwd><kwd>colonization resistance</kwd><kwd>intercellular matrix</kwd><kwd>fluorescence</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Авторы благодарят Московский государственный университет пищевых производств (МГУПП) и Российский университет дружбы на- родов (РУДН) за предоставленные возможности для проведения исследовательской работы</funding-statement><funding-statement xml:lang="en">The authors are grateful to the Moscow State University of Food Production (FGBOU VO MGUPP) and Peoples’ Friendship University of Russia (RUDN) for provided opportunity to perform the research activities</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Джупина С. 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