veterinaryВетеринария сегодняVeterinary Science Today2304-196X2658-6959"Veinard"10.29326/2304-196X-2020-3-34-228-238veterinary-506Research ArticleОБЩИЕ ВОПРОСЫGENERAL ISSUESИсследование лечебного воздействия вод термальных источников провинции Афьон на экспериментально индуцированную жировую дистрофию печени у мышейInvestigation of the healing effects of Afyon Province hot spring waters on experimentally-induced fatty liver in micehttps://orcid.org/0000-0003-3336-4479ElitokBülentElitokBülent

Bülent Elitok, доцент, доктор наук, кафедра терапии, факультет ветеринарной медицины

03200, г. Афьонкарахисар

Bülent Elitok, Associate Professor, Doctor of Science, Department of Internal Medicine, Faculty of Veterinary Medicine

03200, Afyonkarahisar

elitok1969@hotmail.comKişlalioğluIbrahimKişlalioğluIbrahim

Ibrahim Kişlalioğlu, магистрант, ветеринарный врач, управление сельского хозяйства провинции

г. Испарта

Ibrahim Kişlalioğlu, Master Student, Veterinarian, Provincial Directorate of Agriculture

Isparta

UlusoyYavuzUlusoyYavuz

Yavuz Ulusoy, доктор наук, заведующий лабораторией патологии

г. Анкара

Yavuz Ulusoy, Doctor of Science, Head of Pathology Laboratory

Ankara

yavuz.ulusoy@tarim.gov.trKilinçBahadırKilinçBahadır

Bahadır Kilinç, ветеринарный врач лаборатории патологии

г. Анкара

Bahadır Kilinç, Veterinarian, Pathology Laboratory

Ankara

bahadir.kilinc@tarim.gov.trУниверситет Афьон КоджатепеТурцияAfyon Kocatepe UniversityTurkeyМинистерство сельского и лесного хозяйства, управление сельского хозяйства провинцииТурцияMinistry of Agriculture and ForestryTurkeyЦентральный научно-исследовательский институт ветеринарного контроля, Министерство сельского и лесного хозяйстваТурцияVeterinary Control Central Research Institute, Ministry of Agriculture and ForestryTurkey20201609202003228238Copyright © Elitok B., Kişlalioğlu I., Ulusoy Y., Kilinç B., 20202020Elitok B., Kişlalioğlu I., Ulusoy Y., Kilinç B.Elitok B., Kişlalioğlu I., Ulusoy Y., Kilinç B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://veterinary.arriah.ru/jour/article/view/506

В связи с возрастающим количеством случаев развития алкогольной жировой дистрофии печени в мире актуальной задачей является разработка методов лечения этого заболевания. Из литературных данных известно, что минеральная вода из термальных источников оказывает благотворное влияние на клетки печени. В связи с этим было проведено исследование по оценке эффективности применения термальной воды из источника Süreyya I (провинция Афьонкарахисар) при лечении жировой дистрофии печени. Для эксперимента были отобраны 50 мышей-альбиносов суточного возраста со средней массой тела 29,6 г. Через шесть недель применения этилового спирта у мышей сформировалось ожирение печени. Проведенные на этом этапе исследования тканей печени, биохимический и гематологический анализы крови, а также анализ газового состава демонстрировали ухудшение всех показателей. Для доказательства эффективности применения термальной воды при лечении алкогольной жировой дистрофии печени были сформированы две группы по 25 мышей в каждой. Животных контрольной группы выпаивали водопроводной водой, а также устраивали из нее ежедневные часовые ванны. Мышей опытной группы поили термальной водой, а также купали в ней по 15 мин каждый день. Гистологические исследования и анализы крови проводили на 1, 7, 14 и 21-е сут эксперимента у 5 произвольно выбранных из каждой группы животных. На 21-е сут исследования у животных опытной группы наблюдалось достоверное (р < 0,05) снижение общего количества лейкоцитов, нейтрофилов, моноцитов, а также уровней аспартатаминострансферазы, аланинаминострансферазы, гамма-глутамилтрансферазы, липопротеинов низкой плотности, концентрации общего холестерина, триглицеридов. Также наблюдалось увеличение уровней эритроцитов, гемоглобина, гематокрита, общего белка, альбумина и липопротеинов высокой плотности (р < 0,05). Положительная динамика наблюдалась также по результатам гистопатологического анализа. В то же время у животных контрольной группы ярко выраженной положительной динамики не наблюдалось. Более того, микроскопия проб печени показала продолжающийся процесс дегенерации тканей. Полученные данные позволяют сделать вывод о целесообразности применения термальной воды для лечения алкогольной жировой дистрофии печени.

Due the increasing number of alcoholic fatty liver disease cases in the world, the development of methods for treating this disease is an urgent task. According to different publications mineral water from hot springs has a beneficial effect on liver cells. In this regard, an investigation was performed with the aim to assess the effectiveness of hot spring water from the Süreyya I spring (Afyonkarahisar province) in treatment of fatty liver disease. 50 one-day-old albino mice with an average body weight of 29.6 g were selected for the experiment. The tests of liver tissue, biochemical and hematological blood tests, as well as blood gas tests performed at this stage, demonstrated deterioration in all parameters. To prove the effectiveness of using hot spring water in the treatment of alcoholic fatty liver disease, two groups of 25 mice each were formed. The animals of the control group were given tap water to drink, and were also bathed (daily) in it for one hour. The mice of the experimental group were given the hot spring water to drink and bathed in it for 15 minutes every day. Histological tests and blood tests were performed on day 1, 7, 14, and 21 of the experiment using five animals randomly selected from each group. On day 21 of the experiment, the animals of the experimental group demonstrated a significant (p < 0.05) decrease in the total number of leukocytes, neutrophils, monocytes, as well as in the levels of aspartate aminostatesferase, alanine aminopenesferase, gamma-glutamyltransferase, low-density lipoproteins, total cholesterol, triglycerides. There was also an increase in erythrocyte, hemoglobin, hematocrit, total protein, albumin and high density lipoprotein levels (p < 0.05). The results of histopathological analysis also demonstrated positive dynamics. At the same time, no pronounced positive dynamics was observed in animals of the control group. Moreover, microscopy of liver samples showed an ongoing process of tissue degeneration. The data obtained allow us to conclude that it is advisable to use the hot spring water for the treatment of alcoholic fatty liver disease.

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The authors declare that there are no conflicts of interest present.