veterinaryВетеринария сегодняVeterinary Science Today2304-196X2658-6959"Veinard"veterinary-190Research ArticleСтатьиКлонирование и экспрессия в E. COLI генов К205 R и B602L вируса африканской чему свинейCloning and expression of African swine fever virus K205R and B602L genes inf. COLIЩербаковА. В.ScherbakovA. V.noemail@neicon.ruЯковлеваА. С.YakovlevaA. S.noemail@neicon.ruТиминаА. М.TiminaA. M.noemail@neicon.ruЯкуповМ. Р.YakupovM. R.noemail@neicon.ruФГБУ «ВНИИЗЖ», г. ВладимирRussian Federation201502042018022731Copyright © Щербаков А.В., Яковлева А.С., Тимина А.М., Якупов М.Р., 20182018Щербаков А.В., Яковлева А.С., Тимина А.М., Якупов М.Р.Scherbakov A.V., Yakovleva A.S., Timina A.M., Yakupov M.R.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://veterinary.arriah.ru/jour/article/view/190

Проведено молекулярное клонирование генов K205R и B602L вируса африканской чумы свиней в f. coli. Отработаны условия экспрессии и очистки, обеспечивающие высокий выход рекомбинантныхбел ков. Очистку растворенных рекомбинантных бел ков проводили методом металл-хелатной хроматографии с применением Ni-NTA-agarose («Qiagen»). Рекомбинантные антигены биологически безопасны, более технологичны в приготовлении и обеспечивают более высокую специфичность ИФА. Выход очищенного протеина со 100 мл культуры £ со//составил 1,5 мг для pK205R и 2 мг для pB602L.

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The authors declare that there are no conflicts of interest present.