veterinaryВетеринария сегодняVeterinary Science Today2304-196X2658-6959"Veinard"veterinary-177Research ArticleСтатьиIMPLEMENTATION OF 454 LIFE SCIENCES TECHNOLOGY LABORATORY PRACTICESZinyakovN. G.noemail@neicon.ruOvchinnikovaYe. V.zinyakov@arriah.ruLazarevaS. P.noemail@neicon.ruKozlovA. A.noemail@neicon.ruChvalasI. A.noemail@neicon.ruFGBI "ARRIAH", VladimirRussian Federation201502042018014044Copyright © Zinyakov N.G., Ovchinnikova Y.V., Lazareva S.P., Kozlov A.A., Chvalas I.A., 20182018Zinyakov N.G., Ovchinnikova Y.V., Lazareva S.P., Kozlov A.A., Chvalas I.A.Zinyakov N.G., Ovchinnikova Y.V., Lazareva S.P., Kozlov A.A., Chvalas I.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://veterinary.arriah.ru/jour/article/view/177

He article demonstrates possibility of using highly productive pyrophosphate sequencing, also known as 454 Life Sciences technology for investigation of highly variable and understudied infectious viral agents. Genome-wide nucleotide sequences of infectious bronchitis virus, infectious laryngotracheitis virus, fowl adenovirus C, and avian adenoassociated virus were determined. Recombinations in infectious bronchitis genome were identified, structural and point mutations in fowl adenovirus genome were detect ed. Infectious laryngotracheitis virus and avian adeno-associated virus genome structure was characterized.

infectious bronchitisinfectious laryngotracheitisfowl adenovirusavian adeno-associated virusgenomenucleic acidssequencingReferencesGenetic analysis of fowl adenovirus isolate «Krasnodar 2009» / O. S. Osipova, N. G. Zinyakov, I. A.Chvala, V. V. Drygin//VestnikVeterinarii. -2014, -№3, -P. 33-37.Genetic analysis of fowl adenovirus isolate «Krasnodar 2009» / O. S. Osipova, N. G. Zinyakov, I. A.Chvala, V. V. Drygin//VestnikVeterinarii. -2014, -№3, -P. 33-37.Zinyakov N. G. Detection of adeno-associated viruses using NGS-techniques//Molecular Diagnostics. - 2014.- Vol. 2, -P. 448-449.Zinyakov N. G. Detection of adeno-associated viruses using NGS-techniques//Molecular Diagnostics. - 2014.- Vol. 2, -P. 448-449.Ovchinnikova Ye. V. Molecular and biological properties of IBV isolates detected in Russia in 2005-201Ί: thesis.. candidate of science (Biology).- Vladimir, 2012. -116 p.Ovchinnikova Ye. V. Molecular and biological properties of IBV isolates detected in Russia in 2005-201Ί: thesis.. candidate of science (Biology).- Vladimir, 2012. -116 p.Adair В. М., Fitzgerald S. D. Group 1 adenovirus infections // Diseases of Poultry / ed. Y. M. Saif, A. M. Fadly, J. R. Glisson [et al.].- 12th ed. - Ames, Iowa, - 2008.- P. 252-266.Adair В. М., Fitzgerald S. D. Group 1 adenovirus infections // Diseases of Poultry / ed. Y. M. Saif, A. M. Fadly, J. R. Glisson [et al.].- 12th ed. - Ames, Iowa, - 2008.- P. 252-266.Bossis I., Chiorini J. A. Cloning of an Avian Adeno-Associated Virus (AAAV) and Generation of Recombinant AAAV Pa rticles/J. Virol. - 2003,-Vol. 77. - P. 6799-6810.Bossis I., Chiorini J. A. Cloning of an Avian Adeno-Associated Virus (AAAV) and Generation of Recombinant AAAV Pa rticles/J. Virol. - 2003,-Vol. 77. - P. 6799-6810.Knopf C. W., Spies B., Kaerner H. C. The DNA replication origins of herpes simplex virus type 1 strain Angelotti // Nucleic Acids Research.- 1986.-Vol. 14, № 21.- P. 8655-8667.Knopf C. W., Spies B., Kaerner H. C. The DNA replication origins of herpes simplex virus type 1 strain Angelotti // Nucleic Acids Research.- 1986.-Vol. 14, № 21.- P. 8655-8667.Metagenomic analysis of the viromes of three North American bat species: viral diversity among different bat species that share a common habitat / E. F. Donaldson, A. N. Haskew, J. E. Gates [et al.] // J. Virol. - 2010.-Vol. 84. - P. 13004-13018.Metagenomic analysis of the viromes of three North American bat species: viral diversity among different bat species that share a common habitat / E. F. Donaldson, A. N. Haskew, J. E. Gates [et al.] // J. Virol. - 2010.-Vol. 84. - P. 13004-13018.Sequencing viral genomes from a single isolated plaque / J. DePew, B. Zhou, J. M. McCorrison [et al.] // J. Virol. - 2013. - Vol. 10 (181 ). - doi: 10.1186/1743-422X-10-181.Sequencing viral genomes from a single isolated plaque / J. DePew, B. Zhou, J. M. McCorrison [et al.] // J. Virol. - 2013. - Vol. 10 (181 ). - doi: 10.1186/1743-422X-10-181.

The authors declare that there are no conflicts of interest present.