veterinaryВетеринария сегодняVeterinary Science Today2304-196X2658-6959"Veinard"10.29326/2304-196X-2026-15-2-164-169veterinary-1016Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ | БОЛЕЗНИ МЕЛКИХ ДОМАШНИХ ЖИВОТНЫХORIGINAL ARTICLES | DISEASES OF SMALL PETSВыделение вируса инфекционного ринотрахеита кошек и его антигенные свойстваIsolation of feline viral rhinotracheitis agent and its antigenic propertieshttps://orcid.org/0000-0001-9494-8537ГалкинаТ. С.GalkinaT. S.

Галкина Татьяна Сергеевна, канд. вет. наук, заведующий лабораторией профилактики болезней мелких домашних животных

ул. Гвардейская, 6, мкр. Юрьевец, г. Владимир, 600901

Tatyana S. Galkina, Cand. Sci. (Veterinary Medicine), Head of Laboratory for Pets Disease Prevention

ul. Gvardeyskaya, 6, Yur’evets, Vladimir 600901

galkina_ts@arriah.ruhttps://orcid.org/0000-0002-1876-2025КомароваА. А.KomarovaA. A.

Комарова Анна Александровна, ведущий ветеринарный врач лаборатории профилактики болезней мелких домашних животных

ул. Гвардейская, 6, мкр. Юрьевец, г. Владимир, 600901

Anna A. Komarova, Leading Veterinarian, Laboratory for Pets Disease Prevention

ul. Gvardeyskaya, 6, Yur’evets, Vladimir 600901

komarova_aa@arriah.ruhttps://orcid.org/0000-0003-3921-8050КиселевА. М.KiselevA. M.

Киселев Алексей Максимович, канд. вет. наук, ветеринарный врач лаборатории профилактики болезней мелких домашних животных

ул. Гвардейская, 6, мкр. Юрьевец, г. Владимир, 600901

Alexey M. Kiselev, Cand. Sci. (Veterinary Medicine), Veterinarian, Laboratory for Pets Disease Prevention

ul. Gvardeyskaya, 6, Yur’evets, Vladimir 600901

kiselev_am@arriah.ruhttps://orcid.org/0009-0006-9544-5898ЭлизбарашвилиЭ. И.ElizbarashviliE. I.

Элизбарашвили Элизбар Иосифович, канд. вет. наук, главный специалист отдела вирусологии

Звенигородское шоссе, 5, г. Москва, 123022

Elizbar I. Elizbarashvili, Cand. Sci. (Veterinary Medicine), Chief Specialist, Department of Virology

Zvenigorodskoe shosse, 5, Moscow 123022

elizbarashvili@vgnki.ruФГБУ «Федеральный центр охраны здоровья животных» (ФГБУ «ВНИИЗЖ»)РоссияFederal Centre for Animal HealthRussian FederationФГБУ «Всероссийский государственный Центр качества и стандартизации лекарственных средств для животных и кормов» (ФГБУ «ВГНКИ»)РоссияThe Russian State Centеr for Quality and Standardization of Veterinary Drugs and FeedRussian Federation202621062026152164169Copyright © Галкина Т.С., Комарова А.А., Киселев А.М., Элизбарашвили Э.И., 20262026Галкина Т.С., Комарова А.А., Киселев А.М., Элизбарашвили Э.И.Galkina T.S., Komarova A.A., Kiselev A.M., Elizbarashvili E.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://veterinary.arriah.ru/jour/article/view/1016Введение

Введение. Вирусные респираторные заболевания являются серьезной проблемой для здоровья кошек во всем мире. Герпесвирус кошек 1-го типа (Feline herpesvirus type 1, FHV-1) – возбудитель высококонтагиозного заболевания, вызывающий инфекции верхних дыхательных путей, а также язвы конъюнктивы и роговицы. Герпесвирус 1-го типа считается основным этиологическим агентом респираторных инфекций у кошек, и, по оценкам специалистов, около 50–70% случаев респираторных инфекций связаны с данным возбудителем.

Цель исследования

Цель исследования. Выделение вируса инфекционного ринотрахеита кошек из патматериала от больных животных в культуре клеток, идентификация и изучение его антигенных свойств.

Материалы и методы

Материалы и методы. Для выделения вируса in vitro использовали первично трипсинизированную культуру клеток почки эмбриона котенка, затем адаптировали его к перевиваемой культуре клеток почки кошки CRFK. Детекцию возбудителя инфекционного ринотрахеита кошек осуществляли с помощью полимеразной цепной реакции с обратной транскрипцией и полимеразной цепной реакции в режиме реального времени с применением специфичных праймеров, комплементарных участкам амплифицируемых фрагментов комплементарной ДНК. Вируснейтрализующие антитела в сыворотке крови иммунизированных кроликов определяли в реакции нейтрализации с использованием монослойной перевиваемой клеточной линии почки кошки CRFK.

Результаты

Результаты. Установлено, что вирус инфекционного ринотрахеита кошек обнаруживается в конъюнктивальных, назальных, ротоглоточных смывах, реплицируется с проявлением выраженного цитопатического действия в первичной и субкультуре клеток почек эмбриона котенка и перевиваемой культуре клеток почки кошки CRFK, титр инфекционной активности через 72 ч после его инокуляции составлял (6,50 ± 0,25) и (7,40 ± 0,22) lg ТЦД50 /см3 соответственно.

Заключение

Заключение. Полученный в результате исследований изолят герпесвируса кошек 1-го типа, обозначенный как штамм «Лавр», может использоваться для изготовления диагностических препаратов и средств специфической профилактики инфекционного ринотрахеита у кошек.

Introduction

Introduction. Viral respiratory diseases pose a serious problem for the health of cats all over the world. Feline herpesvirus type 1 (FHV-1) is the causative agent of a highly contagious infectious disease that induces upper respiratory tract infections, as well as conjunctiva and cornea ulcers. Feline herpesvirus 1 is considered the main etiological agent of respiratory infections in cats, and, according to experts, about 50–70% of respiratory infection cases are associated with this pathogen.

Objective

Objective. Isolation of feline viral rhinotracheitis agent from pathological material of diseased animals in cell culture; its identification and study of antigenic properties.

Materials and methods

Materials and methods. To isolate the virus in vitro, a trypsinized primary feline embryo kidney cell culture was used, which was subsequently adapted to continuous feline kidney cell line CRFK. The causative agent of feline viral rhinotracheitis was detected by reverse transcription polymerase chain reaction and real-time polymerase chain reaction using specific primers complementary to the amplified complementary DNA fragments. The virus neutralizing antibodies were detected in the sera of immunized rabbits by virus neutralization test using continuous feline kidney cell CRFK monolayer.

Results

Results. It was found that feline viral rhinotracheitis agent is detected in the conjunctival, nasal, and oropharyngeal swabs. It is replicated with the pronounced cytopathic effect in the primary and subcultured feline embryo kidney cells and in continuous feline kidney cell culture CRFK. The infectious activity titre 72 hours post inoculation was (6.50 ± 0.25) and (7.40 ± 0.22) lg TCID50 /mL, respectively.

Conclusion

Conclusion. Feline herpesvirus 1 isolate obtained as a result of the study and designated as Lavr strain, can be used for the manufacture of diagnostic products and means of specific prevention of feline viral rhinotracheitis in cats.

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The authors declare that there are no conflicts of interest present.